高效液相色谱法分析国产妥布霉素及有关物质  

Analysis of tobramycin made in China and related substances by HPLC

作　　者：杨茂俊[1] 吴章秀 

机构地区：[1]国家医药管理局四川抗菌素工业研究所

出　　处：《中国抗生素杂志》1996年第A00期72-76,共5页Chinese Journal of Antibiotics

摘　　要：样品用２，４，６－三硝基苯磺酸在８５℃进行衍生化。反相高效液相色谱法分离、紫外检测器检测，分离、定量了妥布霉素及其有关物质新霉胺、卡那霉素Ｂ、尼伯拉胺及氨甲酰妥布霉素。用本法分析了９批国产妥布霉素样品。A HPLC method for analysis of tobramycin and related substances is described.The sample were derivatized with 2,4,6-trinitrobensulphonic acid at 85℃ for 45 min.Using UV absorbance(340nm) of derivatives for detection. Tobramycin and neamine,kanamycin B, nebramine,carbamoyltobramycin were separated effectively by reverse phase HPLC.

关 键 词：妥布霉素 高效液相色谱 

分 类 号：TQ465.2[化学工程—制药化工]