固定化芽孢杆菌修复油污染地表水的研究  

Study on Bioremediation of Oil-contaminated Surface Water by Using Immobilized Bacillus Sp.

作　　者：张辉[1] 李培军[2] 胡筱敏[1] 王新[1] V.A.Verkhozina 

机构地区：[1]东北大学资源与土木工程学院,辽宁沈阳110004 [2]中国科学院沈阳应用生态研究所,辽宁沈阳110023 [3]俄罗斯科学院西伯利亚分院闵诺格拉多夫地质化学研究所

出　　处：《中国给水排水》2007年第7期28-31,共4页China Water & Wastewater

基　　金：国家高技术研究发展计划(863)项目(2004AA649060);中俄自然资源与生态环境联合研究中心基金资助项目;沈阳市环境工程重点实验室基金资助项目(04002)

摘　　要：采用改进的聚乙烯醇—硼酸包埋固定化微生物法固定芽孢杆菌,并用于处理油污染地表水。结果表明,固定化芽孢杆菌对地表水中油和COD的去除能力均大于游离菌,作用120 h后固定化芽孢杆菌对油的去除率为80.1%,对COD的去除率为79.4%;游离菌对油的去除率为50.4%,对COD的去除率为66.5%。采用改进的聚乙烯醇—硼酸法制得的固定化颗粒机械强度高、传质性能好、水溶膨胀性小,扫描电子显微镜观察表明,改进后的聚乙烯醇凝胶微观结构更适合芽孢杆菌生长。可见,采用微生物固定化技术修复油污染地表水是可行的。The remediation of oil-contaminated surface water using immobilized Bacillus sp. made by the improved PVA - H3BO3 method was studied. The results indicate that the removal effect of immobilized Bacillus sp. on oil and COD in surface water is better than that of free Bacillus sp. The removal rates of oil and COD by immobilized Bacillus sp. after 120 h are 80.1% and 79.4% respectively, and the removal rates of oil and COD by free Bacillus sp. are 50.4% and 66.5% , respectively. The gel bead made by the improved PVA - H3BO3 method has well mechanical intensity and mass transfer characteristics, and a small water-swelling. The scanning electron microscope （SEM） observations show that the gel microstructure is more suitable for the growth of Bacillus sp. That is to say, the remediation of oil-contaminated surface water by using the microbial immobilization technology is feasible.

关 键 词：微生物固定化 芽孢杆菌 生物修复 油污染地表水 

分 类 号：X52[环境科学与工程—环境工程]