Specific Proteins in the Indirect Somatic Embryogenesis of Freesia Refracta  

作　　者：GAO Xiang HUI Yue-jun XUE Yi-qun FU Yang AO Man WANG Li 

机构地区：[1]Institute of Genetics and Cytology,Ministry of Education, Northeast Normal University, Changchun 130024, P. R. China [2]Key Laboratory of Vegetation Ecology, Ministry of Education, Northeast Normal University, Changchun 130024, P. R. China

出　　处：《Chemical Research in Chinese Universities》2007年第2期187-190,共4页高等学校化学研究（英文版）

基　　金：the National Natural Science Foundation of China(No 30570170);Science and Technology Department of Jinlin Province, P.R.China(No 20050218);Jingyue Zone of Changchun City, P.R.China(No 2005D007) ;the Program for Changjiang Scholars and Innovative Research Team(PCSIRT) in Universities of P. R.China

摘　　要：Using the young inflorescence segments of Freesia refracta as explants, indirect somatic embryogenesis of somatic cells was induced in a N6 medium supplemented with some exogenous hormones. SDS-polyacrylamide gel electrophoresis（SDS-PAGE） was used for the analysis of soluble proteins produced during the somatic embryogenesis of this plant. There are six polypeptides, which might play some roles in the process of somatic embryo development. Tltree polypeptides（45, 53 and 55 kD） were detected in the stages of embryogenic callus, globular embryoid, and embryoid with coleoptiles, except the embryoid with leaf. One polypeptide（ 83 kD） was specific for the stages of embryoid with eoleoptiles and embryoid with leaf. One polypeptide（37 kD） was detected in the first two stages, namely, embryogenic callus and globular embryoid. One polypeptide（35 kD） was regularly synthesized in each stage, from embryogenic callus to embryoid with leaf.Using the young inflorescence segments of Freesia refracta as explants, indirect somatic embryogenesis of somatic cells was induced in a N6 medium supplemented with some exogenous hormones. SDS-polyacrylamide gel electrophoresis（SDS-PAGE） was used for the analysis of soluble proteins produced during the somatic embryogenesis of this plant. There are six polypeptides, which might play some roles in the process of somatic embryo development. Tltree polypeptides（45, 53 and 55 kD） were detected in the stages of embryogenic callus, globular embryoid, and embryoid with coleoptiles, except the embryoid with leaf. One polypeptide（ 83 kD） was specific for the stages of embryoid with eoleoptiles and embryoid with leaf. One polypeptide（37 kD） was detected in the first two stages, namely, embryogenic callus and globular embryoid. One polypeptide（35 kD） was regularly synthesized in each stage, from embryogenic callus to embryoid with leaf.

关 键 词：Freesia refiacta Somatic embryo Specific protein 

分 类 号：Q946.1[生物学—植物学] Q943.1