海因酶法耦合原位分离技术制备N-氨甲酰-D-苯丙氨酸  

Production of N-carbamoyl-D-phenylalanine by hydantoinase method coupled with in situ product removal

作　　者：徐晓滢[1] 姚忠[1] 马哲[1] 刘辉[1] 周华[1] 韦萍[1] 

机构地区：[1]南京工业大学制药与生命科学学院,江苏南京210009

出　　处：《化工学报》2007年第4期980-986,共7页CIESC Journal

基　　金：国家重点基础研究发展计划项目(2003CB7160004)~~

摘　　要：The purified D-hydantoinase was immobilized on EAH sepharose 4B via the carbodiimide method with a yield of enzyme activity up to 79.44%.The immobilized hydantoinase showed remarkable stability at 4℃.An integrated process of N-carbamoyl-D-phenylalanine(N-D-Phe)synthesis from D,L-5-benzylhydantoin(D,L-BH)catalyzed by immobilized D-hydantoinase coupled with an ion-exchange unit for in situ product removal(ISPR)was established.The variation of pH and conversion in the fixed-bed reactor with or without ISPR was compared at different temperatures,initial substrate concentrations and volumes of adsorbent.Within 24 h,the pH value in the reactor with ISPR could be kept at the alkaline range,which was beneficial to the enzymatic conversion and racemization of L-5-benzyl hydantoinase.This led to a higher overall conversion of 62.725% under optimal operation conditions,an increase of 89.3% compared with the fixed-bed reactor without ISPR.The purified D-hydantoinase was immobilized on EAH sepharose 4B via the carbodiimide method with a yield of enzyme activity up to 79.44 %. The immobilized hydantoinase showed remarkable stability at 4℃. An integrated process of N-carbamoyl-D-phenylalanine （N-D-Phe） synthesis from D,L-5-benzylhydantoin （D, L-BH） catalyzed by immobilized D-hydantoinase coupled with an ion-exchange unit for in situ product removal （ISPR） was established. The variation of pH and conversion in the fixed-bed reactor with or without ISPR was compared at different temperatures, initial substrate concentrations and volumes of adsorbent. Within 24 h, the pH value in the reactor with ISPR could be kept at the alkaline range, which was beneficial to the enzymatic conversion and racemization of L-5-benzyl hydantoinase. This led to a higher overall conversion of 62. 725% under optimal operation conditions, an increase of 89.3% compared with the fixed-bed reactor without ISPR.

关 键 词：固定化 D-海因酶 固定床反应器 原位分离 N-氨甲酰-D-苯丙氨酸 

分 类 号：TQ936.2[化学工程]