抗生素对泡桐丛枝病植原体和发病相关蛋白质的影响  

Effects of Antibiotics on the Paulownia Witches' Broom Phytoplasmas and Pathogenic Protein Related to Witches' Broom Symptom

作　　者：范国强[1] 张胜[1] 翟晓巧[1] 刘飞[1] 董占强[1] 

机构地区：[1]河南农业大学泡桐研究所,郑州450002

出　　处：《林业科学》2007年第3期138-142,共5页Scientia Silvae Sinicae

基　　金：国家自然科学基金(30271082;30571496);高等学校博士学科点专项科研基金(20050466003);河南省杰出青年基金(0612001900)资助项目。

摘　　要：The effects of different concentrations of 5-fluorouracil, rifampicin and tetracycline on the phytoplasmas in the Paulownia tomentosa×Paulownia fortunei seedling and the pathogenic protein related to the witches’ broom symptom were studied with PCR based on 16S rRNA gene of the phytoplasmas and 2-dimensional electrophoresis of proteins. The results indicated that the plantlets in vitro culture treated with 0.5～300 mg·L -1 5-fluorouracil had little effect on the symptom remission, but those plantlets treated with 100 and 150 mg·L -1 rifampicin for 30 d showed obvious effect on the symptom remission respectively, and the phytoplasmas in the plantlets treated with 150 mg·L -1 rifampicin could not be detected with PCR of 16S rDNA of the phytoplasmas. Moreover, the protein (pI6.8,m 24 ku) which appeared in both the healthy seedling and diseases ones treated with 150 mg·L -1 rifampicin disappeared in the infected seedling and the those without the symptom after treating with rifampicin and tetracycline, and in which existed with phytoplasma through PCR detection.The effects of different concentrations of 5-fluorouracil, rifampicin and tetracycline on the phytoplasmas in the Paulowniatomentosa × Paulownia fortunei seedling and the pathogenic protein related to the witches＇ broom symptom were studied with PCR based on 16S rRNA gene of the phytoplasmas and 2-dimensional electrophoresis of proteins. The results indicated that the plantlets in vitro culture treated with 0.5 - 300 mg· L^-1 5-fluorouracil had little effect on the symptom remission, but those plantlets treated with 100 and 150 mg· L^-1 rifampicin for 30 d showed obvious effect on the symptom remission respectively, and the phytoplasmas in the plantlets treated with 150 mg· L^-1 rifampicin could not be detected with PCR of 16S rDNA of the phytoplasmas. Moreover, the protein （p16.8, m 24 ku） which appeared in both the healthy seedling and diseases ones treated with 150 mg· L^-1 rifampicin disappeared in the infected seedling and the those without the symptom after treating with rifampicin and tetracycline, and in which existed with phytoplasma through PCR detection.

关 键 词：泡桐 丛枝病 蛋白质 双向电泳 PCR 

分 类 号：S763.1[农业科学—森林保护学]