HWTX-I对全脑缺血大鼠脑组织自由基及海马神经元损伤的保护作用研究  

Research on Protective Effects of HWTX-I on the Damages of Free Radical and Hippocampus Neurons in the Brain of Cerebral Ischemia Rats

作　　者：毛海峰[1] 陈嘉勤[1] 梁宋平[1] 周鸿雁[1] 王一蓉[1] 刘仁义[1] 张沙骆[1] 

机构地区：[1]湖南师范大学,湖南长沙410012

出　　处：《北京体育大学学报》2007年第3期351-353,共3页Journal of Beijing Sport University

基　　金：湖南省自然科学基金资助课题(04JJ3014)

摘　　要：目的:观察虎纹捕鸟蛛毒素(HWTX-I)对全脑缺血再灌注大鼠海马CA1区锥体细胞Nissl染色形态学变化、脑组织超氧化物歧化酶(SOD)、过氧化氢酶(CAT)活力、丙二醛(MDA)浓度变化的影响,探讨其改善全脑缺血再灌注损伤的机制,为颅脑损伤的临床治疗提供新的有效治疗手段。方法:SD大鼠随机分为假手术组、生理盐水组及三组用药组,采用改良的Pulsinelli四血管阻断全脑缺血损伤模型结合蛛网膜下腔置管术,对大鼠海马锥体细胞进行Nissl染色及测定各组大鼠脑组织SOD、CAT活力及MDA浓度。结果:1)HWTX-I能明显减轻全脑缺血大鼠海马锥体细胞的损伤。2)HWTX-I能使全脑缺血再灌注大鼠脑组织SOD、CAT活力明显升高,MDA浓度明显降低。结论:HWTX-I对全脑缺血再灌注等引起的大鼠大脑神经细胞损伤有明显的保护作用。To observe the effects of Hu Wen Toxin-I（HWTX-I） on morphological changes by Nissl staining of hippocampus CA1 pyramidal neurons of global cerebral ischimia reperfusion rats, activity of SOD, CAT in brain and the content changes of MDA, HWTX-Ⅰ＇s mechanism of relieving global cerebral ischimia reperfusion damage is researched, so as to offer new effective treatment to cranicerebral injury in clinic. SD rats was randomly divided into the false operation group, the physiological saline group and 3 administration groups, by adopting the model of improved Pulsinelli 4-vessals occlusion global cerebral ischemia damages combined with the methods of playing anaesthesic tube into intrathecal space, staining the rats hippocampus CA1 pyramidal neurons and determining all rats SOD, CAT activity and MDA content in brain. The results are as follows： 1. HWTX-Ⅰ can obviously relieve the damage of hippocampns pyramidal neurons in global cerebral ischimia rats. 2. HWTX-Ⅰ can obviously increase the activity of SOD and CAT in global cerebral ischimia rats and decrease MDA content noticeably. Therefore, HWTX-Ⅰ has obviously neuro-pretective effects on rats neurons damages induced by global cerebral ischimia reperfusion.

关 键 词：虎纹捕鸟蛛毒素(HWTX-Ⅰ) 全脑缺血再灌注 超氧化物歧化酶(SOD) 过氧化氢酶(CAT) 丙二醛 (MDA) 

分 类 号：G804.7[文化科学—运动人体科学]