低镁对肉仔鸡红细胞体外氧化应激敏感性及红细胞膜流动性的影响  

Effects of low-magnesium on susceptibility of erythrocyte to in vitro oxidative stress and erythrocyte membrane fluidity in broiler chickens

作　　者：刘永祥[1] 呙于明[1] 

机构地区：[1]中国农业大学动物科学技术学院,北京100094

出　　处：《中国农业大学学报》2007年第2期15-20,共6页Journal of China Agricultural University

基　　金：国家自然科学基金资助项目(30371043)

摘　　要：为研究镁对肉仔鸡红细胞体外氧化应激的敏感性和红细胞膜流动性的影响,本试验用96只1日龄的AA肉仔鸡随机分配为低镁日粮组(镁含量1.2 g/kg)和对照组(镁含量2.4 g/kg)。在体外氧化应激反应体系中,低镁组肉仔鸡红细胞丙二醛(MDA)的生成量高于对照组(P<0.01),低镁日粮显著提高红细胞膜的各向异性参数(P<0.05);低镁组肉仔鸡红细胞的谷胱甘肽(GSH)含量降低了17%,丙二醛(MDA)的含量升高了19%(P<0.05),红细胞膜的镁含量降低18%(P<0.05);低镁日粮组肉仔鸡红细胞膜胆固醇的含量(P<0.05)及胆固醇/磷脂比(P<0.01)均显著提高。结果表明表明低镁日粮削弱了红细胞的抗氧化系统从而提高红细胞对体外氧化应激的敏感性;红细胞膜胆固醇含量的提高和丙二醛(MDA)的积累可能是低镁日粮降低红细胞膜流动性的原因。The objective of this study was to investigate effects of magnesium on susceptibility of erythrocyte to in vitro oxidative stress and membrane fluidity in broiler chickens, Ninety six 1-day-old male Abor Acre broiler chicks were randomly allocated into low-magnesium and control groups, each group had 6 replicates of 8 male birds each, fed either control or low magnesium diets containing about 1.2 g/kg or 2.4 g/kg of diet respectively. The erythrocyte from low-magnesium group exhibited higher MDA production in in vitro oxidative stress reaction system than that from control （ P 〈 0.05）. The anisotropy parameter of erythrocyte membranes was increased by low magnesium diet （ P 〈 0.05）. Glutathione （GSH） content of erythrocyte decreased by 17% （P〈0.05） while malondialdehyde （MDA） concentration increased by 19% in low-magnesium group （P〈 0.05）. Magnesium content of erythrocyte membrane from low-magnesium group was reduced by 14% （P〈 0.05）. Feeding low-magnesium diet for 21 days resulted in a smaller but significant increase in cholesterol content of erythrocyte membrane （P 〈 0.05） and in higher cholesterol/phospholipids ratio （P〈0.01）. These results suggest that low magnesium diet impaired antioxidant capacity of erythrocyte and then increased susceptibility of erythrocyte to in vitro oxidative stress. Increased MDA content and altered cholesterol composition of erythrocyte membrane should be contributing factors to the decrease of erythrocyte membrane fluidity.

关 键 词：低镁 红细胞 氧化应激敏感性 细胞膜流动性 肉仔鸡 

分 类 号：S831.4[农业科学—畜牧学]