PCR-SSCP法检测结核分枝杆菌L型rpsL耐药基因  

Detection of rpsL drug-resistant gene in L-form of Mycobacterium tuberculosis by PCR-SSCP

作　　者：陆军[1] 叶松[1] 金强[2] 

机构地区：[1]安徽理工大学医学院,安徽淮南232001 [2]蚌埠市第三人民医院检验科,安徽蚌埠233000

出　　处：《临床检验杂志》2007年第2期107-108,共2页Chinese Journal of Clinical Laboratory Science

基　　金：安徽省教育厅自然科学基金项目(2005KJ238);2005年安徽理工大学青年科学研究基金

摘　　要：目的探索尘肺结核患者感染的结核分枝杆菌(MT)L型的耐药基因rpsL突变与耐受链霉素(SM)的关系。方法用PCR-SSCP方法检测rpsL基因,与采用常规SM药敏试验(AST法)的结果进行对比分析。结果采用AST法检测,52株结核分枝杆菌L型临床分离株中共有26株(50.0%)耐SM;PCR-SSCP法检出rpsL基因突变率为40.4%(21/52),2种方法检测耐药株的符合率为80.8%。结论结核杆菌L型对SM的耐药与rpsL基因突变有关。Objective To study the relationship between mutation of rpsL drug-resistant gene in L-forms of Mycobacterium tuberculosis and drug-resistance to streptomycin in pneumoconiosis patients. Methods A total of 52 clinical isolated strains of Mycobacterium tuberculosis L-forms were collected from 97 pneumoconiosis patients. The mutation of rpsL gene was detected by PCR-SSCP,and the drug-resistance to streptomycin was performed by routine antimicrobial susceptibility test （AST）. Results The results of drug susceptibility test showed that 26 in the 52 clinical isolated strains were drug-resistant to streptomycin. The streptomycln-resistant rate was 50.00% （26/52）. The gene mutation rate of rpsL detected by PCR-SSCP was 40. 38% （21/52）. The coincidence rate of two experimental resuits was 80.77% （21/26）. Conclusion High detectable rate of streptomycin-reslstant strains in Mycobacterium tuberculosis L-forms was found by PCR-SSCP. The application of PCR-SSCP may possess important value for guiding clinical medication of pneumoconiosis patients complicated with tuberculosis among coal workers

关 键 词：尘肺 结核分枝杆菌L型 耐药 RPSL基因 聚合酶链反应-单链构象多态性分析 

分 类 号：Q52[生物学—生物化学]