黔北麻羊遗传结构的RAPD分析  

Rapd Analysis of the Genetic Structure of Qianbei-Pockmarked Goat Population

作　　者：陈祥[1] 张芸[2] 廖正录[2] 张勇[1] 李国红[3] 李诗举[1] 王学伟[1] 简承松[1] 魏泓[4] 

机构地区：[1]贵州大学动物科学学院,贵阳550025 [2]贵州省农业厅畜禽品种改良站,贵阳550001 [3]贵州师范大学生物系,贵阳550001 [4]第三军医大学实验动物中心,重庆400038

出　　处：《西南大学学报（自然科学版）》2007年第2期53-56,共4页Journal of Southwest University(Natural Science Edition)

基　　金：国家自然科学基金资助项目(30070120);贵州省科学技术基金[黔科合J字(2005)2031号];[黔基合计字(2002)305号]

摘　　要：用27条多态性引物对15份黔北麻羊个体基因组进行RAPD分析.结果共扩增出253条带,其中多态带为141条,多态频率在30·00%～75·00%之间,平均多态频率为55·73%.单个引物获得的扩增带数在2～14条之间,平均每条引物扩增出9·37条带.扩增带分子量范围在210～2700bp.Nei氏公式计算品种内个体间遗传相似性指数在0·7290～0·9377之间,平均值为0·8634.遗传距离指数在0·0623～0·2710之间,平均值为0·1367.结果表明黔北麻羊具有较丰富的遗传结构.Twenty-seven polymorphic primers were used to amplify the genome DNA of 15 Qianbei-Pockmarked goats, and 253 bands were detected, of which 141 were polymorphic with a polymorphism frequency range of 30.00%~75.00%, averaging 55.73%. Each primer amplified 2~14 bands, averaging 9.37. The fragment length of the amplified products ranged between 210 and 2 700 bp. Calculated by Nei formulae, the genetic similarity index ranged from 0.729 0 to 0.937 7, averaging 0.863 4, and the genetic distance index was between 0.062 3 and 0. 271 0, averaging 0. 136 7. These results indicate that Qianbei-Pockmarked goats have high inter-individual genetic variation and abundant genetic structures.

关 键 词：黔北麻羊 遗传多样性 随机扩增多态脱氧核糖核酸 

分 类 号：S827[农业科学—畜牧学]