平痫冲剂对戊四唑致痫大鼠颞叶谷氨酸受体和γ-氨基丁酸受体的影响  

Effect of Pingxian Powder on N-methyl-D-aspartate Recptor and γ-aminobutyric acid-A Receptor in the Temporal Cortex of Pentylenetetrazole Kindling Epileptic Rat Model

作　　者：张士卿[1] 田茸[1] 史正刚[1] 

机构地区：[1]甘肃中医学院,甘肃兰州730000

出　　处：《中国中医药信息杂志》2007年第4期29-30,共2页Chinese Journal of Information on Traditional Chinese Medicine

基　　金：甘肃省教育厅科研项目(0505-04)

摘　　要：目的探讨平痫冲剂抗癫痫的作用机制。方法腹腔注射戊四唑制成慢性癫痫模型,通过免疫组织化学方法和显微图像分析技术检测大鼠颞叶的谷氨酸受体(NMDAR1)和γ-氨基丁酸受体(GABA-ARα1)免疫反应的阳性细胞数(n)、阳性细胞面积比(Aa%)及阳性细胞积分吸光度(IA)。结果对于NMDAR1,模型组(B组)同正常对照组(A组)比较,颞叶NMDAR1的n、Aa%上升,阳性细胞积分吸光度(IA)增加(P<0.01);而西药治疗组(C组)、中药组(D组)与B组比较,颞叶的NMDAR1的n、Aa%都有不同程度的下降,IA也有不同程度的减少(P<0.01,P<0.05)。对于GABA-ARα1,B组同A组比较,颞叶GABA-ARα1的n、Aa%下降,IA降低(P<0.01);而C组、D组与B组比较,颞叶的GABA-ARα1的n、Aa%都有不同程度的增加,IA也有不同程度的升高(P<0.01,P<0.05)。结论平痫冲剂抗癫痫的作用机制可能是通过抑制颞叶兴奋性;经递质受体NMDAR1的活性与表达,并激活其抑制性;经递质受体GABA-ARα1的活性与表达,从而降低大脑皮质的兴奋性,有效抑制癫痫的形成及发展。Objectives To explore the possible antiepfleptic mechanism of Pingxian Powder. Methods In the experiment, Pentylenetetrazole （PTZ） was injected into abdomen of rat to make epileptic kindling model. The total number, percentage of positive area and integral optical density of N-methyl-D-aspartate receptor1 （NMDAR1） and γ-aminobutyric acid-A receptor （GABA-ARα1） of immunoreactive cells in the temporal cortex were studied by the immunohistochemical method and image analyzing system. The total number, percentage of positive area and integral optical density of NMDAR1 of immunoreactive cells in the temporal cortex of model group were more than normal group （P 〈0.0 1）, while these numerical values of westem and chinese medicine group decreased to varying degrees compared with model group （P〈0.01, P〈0.05）. The total number, percentage of positive area and integral optical density of GABA-ARα1 of immunoreactive cells in the temporal cortex of model group were less than nomal group （P〈0.01）, while these numerical values of westem and chinese medicine group enhanced to varying degrees constrasting with model group （P〈0.01, P〈0.05）. Conclusions Pingxian Powder may reduce the excitability of cerebral cortices by inhibiting the activities of NMDAR1 and promoting the activities of GABA-AR α 1 in the temporal cortex to inhibit the formation and development of epilepsy.

关 键 词：癫痫 平痫冲剂 戊四唑点燃型癫痫大鼠模型 受体 

分 类 号：R289.11[医药卫生—方剂学]