高效液相色谱法测定六味地黄产品中马钱苷含量  

Determination of Loganin in Productions of Liuweidihuang by HPLC

作　　者：景明[1] 樊秦[1] 

机构地区：[1]甘肃中医学院,甘肃兰州730000

出　　处：《中国中医药信息杂志》2007年第4期59-60,共2页Chinese Journal of Information on Traditional Chinese Medicine

摘　　要：目的测定六味地黄产品中马钱苷的含量。方法色谱柱:shim-packvp ODS-C18(4.6mm×150mm,5μm),流动相:四氢呋喃-乙腈-甲醇-0.05%磷酸(1∶8∶4∶87),检测波长:236nm,柱温:40℃,流速:1.0mL/min。结果线性范围:4.42×10-2～22.1×10-2μg,相关系数r=0.9997,平均加样回收率98.96%(n=6,RSD=1.42%)。结论该方法为评价和控制六味地黄系列产品的质量提供了依据。Objective To determine loganin in productions of Liuweidihuang. Methods The chromatographic column was shim-packvp ODS-C18 （4.6 mm× 150mm, 5 μm）, the mobile phase was THF-acetonitrile-methanol-0.05%H4PO3 （1 ： 8 ： 4 ： 87）, the detection wavelength was 236 nm, the temperature of column was 40 ℃ and the flow rate was 1.0 mL/min. Results A good linearity of loganin was in the range of 4.42×10^-2 -22.1× 10^-2 μg, r=0.9997, and the average recovery rate was 98.96% （n=6, RSD= 1.42%）. Conclusion The method provided a reference for evaluating and controlling quality of series productions of Liuweidihuang.

关 键 词：六味地黄产品 马钱苷 高效液相色谱 含量测定 

分 类 号：R284.1[医药卫生—中药学]