玻璃基质微流控芯片用于DNA片段的分离和C677T基因突变的快速检测  

Applications of Glass Microfluidic Chip to Separation of DNA Fragments and Fast Detection of C677T Mutation in Methylenetetrahydrofolate Reductase Gene

作　　者：曲晓峰[1] 任吉存[1] 

机构地区：[1]上海交通大学化学化工学院,上海200240

出　　处：《分析科学学报》2007年第2期153-156,共4页Journal of Analytical Science

基　　金：国家自然科学基金重点项目(No.20335020)

摘　　要：基于微加工方法,在一般实验条件下,成功地制备了玻璃芯片。利用水溶性聚合物对玻璃通道进行动态修饰,从而抑制了DNA分子的吸附作用,并成功地用于DNA片段的分离和四氢叶酸还原酶基因(MTHFR)中C677T的突变检测。In this study,a simple procedure was developed to fabricate glass microfluidic chips. Water-soluble polymers, PDMA and PVP, were used for dynamic coating of glass channels to limit adsorption of DNA molecules. The performance of the coated chips was evaluated by electrophoresis separation of ФX174 HaeⅢ digest DNA fragments. It was found that PDMA as sieving matrix was more effective than PVP in separation of DNA fragments. The glass microchips were successfully applied for the detection of C677T mutation in methylenetetrahydrofolate reductase gene.

关 键 词：玻璃微流控芯片 聚N N-二甲基丙烯酰胺 聚乙烯吡咯烷酮 四氢叶酸还原酶基因的C677T突变 激光诱导荧光检测 

分 类 号：O657.38[理学—分析化学] Q523[理学—化学]