腺病毒介导凋亡素基因转染对人胆管癌细胞Bcl-2蛋白表达的影响  

Bcl-2 protein expression in human bile duct cancer cells transfected by adenoviral vector containing apoptin gene

作　　者：陈健[1] 李大江[1] 王曙光[1] 

机构地区：[1]第三军医大学西南医院全军肝胆外科研究所,重庆400038

出　　处：《西南国防医药》2007年第2期149-152,共4页Medical Journal of National Defending Forces in Southwest China

基　　金：重庆市科委资助项目(2002012)

摘　　要：目的:初步探讨在凋亡素基因转染引发人胆管癌细胞凋亡的同时对Bcl-2蛋白的影响。方法:采用含凋亡素基因的重组腺癌毒感染人胆管癌细胞株QBC939,通过光镜、荧光观察其凋亡情况,在感染后7d收集细胞,采用Western-blot检测其Bcl-2蛋白情况。结果:含凋亡素基因的重组腺病毒感染胆管癌细胞株QBC9397d后其Bcl-2蛋白的表达较空病毒组和空白对照组明显降低。结论:凋亡素基因在诱导人胆管癌细胞凋亡时会导致Bcl-2蛋白的降低,可能有助于凋亡素的作用。To study the expression of Bcl - 2 protein in human bile duct cancer ceils translated by apoptin gene in vitro mediated by adenovirus.Methods：After the human bile duct cancer cell line-QBC 939 was transfected by recombinant adenovirus carrying apoptin gene,the apoptosis of human bile duct cancer cell line QBC 939 was observed under microscope and fluorescence microscope.The expression of Bcl-2 protein was determined with Western blot in the seventh day.Results：The expression of Bcl-2 protein in human bile duct cancer cells transfected by apoptin gene mediated by adenovirus was lower significantly than that in human bile duct cancer cells infected by adenovirus and the control group.Conclusion：Apoptin gene may enhance the apoptosis of bile duct cancer cells by down-regulation of expression of Bcl-2 protein.

关 键 词：凋亡素基因 腺病毒载体 胆管癌细胞 BCL-2蛋白 

分 类 号：R977.11[医药卫生—药品]