促红细胞生成素减轻窒息新生儿血清诱导人肾小管细胞损伤  

Role of Erythropoietin in Relieving Injury of Human Renal Tubular Cell Induced by Postasphyxial-Serum of Neonates

作　　者：熊涛[1] 董文斌[1] 王明勇[2] 邓存良[2] 许开桂[1] 

机构地区：[1]泸州医学院附属医院新生儿科,四川泸州646000 [2]泸州医学院附属医院传染与免疫研究室,四川泸州646000

出　　处：《实用儿科临床杂志》2007年第5期336-337,353,共3页Journal of Applied Clinical Pediatrics

基　　金：四川省杰出青年学科带头人培养计划项目资助(04ZQ026-033)

摘　　要：目的研究促红细胞生成素(EPO)对新生儿窒息后血清导致人近曲肾小管上皮细胞(HK-2)损伤的保护作用。方法以HK-2为研究对象,分为对照、窒息和EPO干预组(每组n=8),以200mL/L窒息血清作为攻击因素,观察各组细胞形态、LDH漏出率和细胞存活[四甲基偶氮唑蓝(MTT)法]变化。结果与对照组比较,窒息组细胞形态发生改变,LDH漏出率增加,细胞存活减少,差异有显著性(P<0.05);而与窒息组比较,除EPO1IU/mL组外,EPO组细胞形态明显得到改善,LDH漏出率降低,细胞存活增加,差异具有显著性(P<0.05),且具有剂量依赖性。结论EPO有减轻窒息后血清所致HK-2细胞损伤作用。Objective To investigate the role of erythropoietin（EPO） in relieving the injury of human renal tubular cells （ HK - 2） induced by postasphyxial - serum of neonates. Methods Human renal proximal tubular cell （ HK - 2 ） was used as the target cell. The experi- ment was designed as control group, asphyxia group, and group of pretreatment with EPO. The attacking concentration of serum was 200 mL/ L, then the changes of morphology were observed under inverted microscope ,and the cell viability was measured by 3 - （4,5 -dimethy hhiazel - 2 - yl） - 2,5 - diphenyl - tetazolium bromide（MTY） methods, and the leakage rate of lactate dehydrogenase （ LDH ） was determined by biochemical methods. Results Compared with control group, the change in morphology of HK -2 was most serious and obvious, and the leakage rate of LDH increased significantly,and the cell viability decreased obviously in asphyxia group. But compared with asphyxia group,the change in morphology of HK - 2 was obviously improved, and the leakage rate of LDH decreased and the cell viability increased in group of pretreatment with EPO in a dose - dependent manner except the group of 11U/mL. Conclusion EPO can play the role in relieving the injury of renal tubular cells induced by postasphyxial - serum in neonates.

关 键 词：促红细胞生成素 窒息 血清 肾小管上皮细胞 肾损伤 婴儿 新生 

分 类 号：R722.1[医药卫生—儿科]