急性肾小球肾炎与肾病综合征患儿体液免疫指标比较  

Comparison of Humoral Immunity Indexes of Acute Glomerulonephritis and Nephritic Syndrome in Children

作　　者：罗晓菊[1] 刘雪梅[1] 豆虎[1] 

机构地区：[1]重庆医科大学附属儿童医院临床免疫室,重庆400014

出　　处：《实用儿科临床杂志》2007年第5期363-364,共2页Journal of Applied Clinical Pediatrics

摘　　要：目的检测急性链球菌感染后肾小球肾炎与肾病综合征(NS)患儿体液免疫指标,进行比较、分析,以达到早期鉴别诊断、早期合理用药目的。方法对23例链球菌感染后肾小球肾炎、48例NS、正常对照18例儿童检测血IgG、IgA、IgM、C3、C4水平,采用英国MININEPH仪器及配套试剂,散射比浊法检测。IgE定量检测采用美国E&ELABSINC公司全套试剂盒,ASO采用免疫比浊法,血沉采用魏氏法检测。结果急性肾小球肾炎与正常对照组比较,IgG、IgA、IgE、C3、C4有显著性差异(Pa<0.05),ASO、血沉具有均明显升高(Pa<0.05)。NS与正常对照组比较,IgG、IgM、IgE具有显著性差异(Pa<0.05);急性肾小球肾炎与NS比较,IgG、IgA、C3、C4具有显著性差异(Pa<0.05),前者IgG、IgA明显升高,C3、C4明显降低,而后者IgG则明显降低。结论急性肾小球肾炎与NS患儿临床表现不典型者可通过检测血IgG、IgA、C3、C4水平加以鉴别,再辅以ASO、血沉检测,可达到早期诊断、早期治疗的目的。Objective To compare the humoral immunity features of acute glomerulonephritis and nephritic syndrome （NS） in children for earlier differential diagnosis and rational administration. Methods Nephelometry was used to determinate the serum level of immunoglobulin （Ig） G, IgA, IgM, C3, C4 in children by England MININEPH machine and correlated reagent. The serum level of IgE was determined by IgE enzyme linked immunosorbent assay（ELISA） ,assay ELISA kit of America E＆ELABSINC company. Immunoturbidimetry and westergren method were used to detect anti -streptolysin O（ASO） and erythroeyte sedimentation rate（ESR） ,respectively. Results There were significant difference features of humoral immunity between acute glomerulonephritis and NS in children, lgG, IgA, IgE, C3 , C4 had significant difference between acute glomerulonephritis children and healthy children （Pa 〈 0.05 ）. IgG, IgM, IgE had significant difference between nephritic syndrome children and healthy children （Pa 〈 0.05）. IgG, IgA, C3 , C4 had significant difference between acute glomerulonephritis and NS children（ Pa 〈 0. 05 ）. IgG, IgA significantly increased, and C3 , C4 significantly decreased in acute glomerulonephritis children. The levels of IgG significantly decrease in NS children. ASO and erythrosedimentation levels both signifieanly increased between acute glomerulonephritis children and healthy children （ Pa 〈 0.05 ）. Conclusion Serum leve of IgG, IgA, C3 , C4 can be used to differentiate atypical acute glomerulonephritis and NS, and diagnose its earlier aid by determinating ASO and erythrosedimentation.

关 键 词：肾小球肾炎 急性 肾病综合征 抗体生成 儿童 

分 类 号：R726.91[医药卫生—儿科]