胆囊疾病的临床快速诊断红外光谱新方法研究  

Fourier Transform Mid-infrared Spectroscopy(FTIR) Used for the Rapid Intraoperative Diagnosis of Gallbladder Diseases

作　　者：徐怡庄[1] 赵莹[2] 凌晓锋[3] 杨丽敏[1] 徐智[3] 周孝思[3] 张元福[1] 龚蓉晔 潘庆华[5] 王冰冰[1] 石景森[6] 徐端夫[2] 吴瑾光[1] 

机构地区：[1]北京大学化学与分子工程学院,稀土材料化学及应用国家重点实验室,北京100871 [2]中国科学院化学研究所,北京100080 [3]北京大学第三医院普通外科,北京100083 [4]北京第二光学仪器厂,北京100015 [5]杭州师范学院及其附属医院,杭州310015 [6]西安交通大学第一医院普通外科,西安710061

出　　处：《高等学校化学学报》2007年第4期645-648,共4页Chemical Journal of Chinese Universities

基　　金：国家重点基础研究发展规划项目(批准号:2002CCA01900);国家自然科学基金(批准号:30371604和50203001);教育部博士学科点基金资助.

摘　　要：研究了衰减全反射(ATR)红外光谱法快速检测胆囊癌的灵敏度和特异性,结果表明,正常组织和胆囊癌组织的红外光谱存在一系列差别,减法光谱技术有助于判别胆囊炎和胆囊癌.统计分析结果表明,此方法灵敏度为100%(6/6),特异性为97%(39/40),准确率为97%(45/46),说明中红外ATR光谱法是一种可快速、无创检测胆囊癌组织的新方法.Identification of gallbladder cancer rapidly at the time of surgery would allow the appropriate resection to be completed at the original surgery. The aim of this study was to assess the sensitivity and specificity of FFIR in the rapid detection of gallbladder cancer, via an attenuated total reflection （ATR） accessory with a ZnSe window. This allowed the edges of the surgically resected specimens to be examined rapidly. The results indicate that there are a series of differences between the spectra of normal and malignant tissues, and subtraction technique is helpful to identify cholecystitis and gallbladder cancer. Compared with pathological diagnosis results, one benign specimen was classified as a malignant and 6/6 malignant specimens and 39 cases of benign specimens were correctly classified. The sensitivity was 100% （6/6）, specificity 97% （39/40）, and accuracy was 97% （45/46）. We can conclude that mid-infrared ATR FFIR spectroscopy is highly accurate and rapidly allows appropriately tailored resection at the time of initial surgery.

关 键 词：傅里叶变换红外光谱法(FTIR) 中红外光纤 胆囊疾病 检测 

分 类 号：O657.33[理学—分析化学]