恒定磁场对Schwann细胞氧化损伤的保护作用  

Protective effects of the invariable magnetic field on oxidative injured Schwann cells

作　　者：张杰[1] 李振华[1] 孙晋浩[1] 暴丽华[1] 刘岳鹏[1] 

机构地区：[1]山东大学医学院解剖学教研室,山东济南250012

出　　处：《山东大学学报（医学版）》2007年第3期229-232,共4页Journal of Shandong University:Health Sciences

基　　金：山东省自然科学基金资助课题(Y2000C14);山东省优秀中青年科学家科研奖励基金资助课题(2004BS02012)

摘　　要：目的:探讨恒定磁场对Schwann细胞氧化损伤保护作用的机制。方法:用体外传代纯化培养的Schwann细胞建立氧化损伤模型,将培养细胞分为氧化损伤组,恒定磁场(4 mT)保护组及正常组。采用四甲基偶氮唑蓝比色法(MTT)检测细胞的活性,用生化技术检测细胞内超氧化物歧化酶(SOD)及丙二醛(MDA)的含量,用免疫组织化学方法检测各组半胱氨酸天冬氨酸酶(Caspase-3)的表达。结果:与正常组及保护组相比,HB2BOB2B处理组细胞活性降低(P<0.01),SOD含量明显减少(P<0.01),MDA含量明显增加(P<0.01),Caspase-3表达呈强阳性,恒定磁场保护组细胞存活率明显升高(P<0.01),SOD含量较损伤组高(P<0.01),MDA含量明显减少(P<0.01),Caspase-3表达弱阳性。结论:4 mT恒定磁场能通过抗氧化损伤保护Schwann细胞。Objective： To investigate the protective effects of the invariable magnetic field on oxidative injured Schwann cells. Methods： Schwann cells were randomly divided into an experimental group and control groups. MTT was used to observe the cell activity of the Schwann cells with hydrogen peroxide. A biologic chemistry method was used to detect the content of intracellular superoxide dismutase （SOD） and malondialdehyde （MDA）. An immunohistochemistry method was used to detect the expression of Caspase-3. Results： The results showed that cell activity decreased in oxidative injured Schwann cells. However, these changes were significantly reversed in the invariable magnetic field preconditioned group. Conclusion： An invariable magnetic field has a good antioxidant effect on Schwann cells.

关 键 词：周围神经系统 模型 神经学 损伤 细胞凋亡 

分 类 号：R322.85[医药卫生—人体解剖和组织胚胎学]