bFGF和TNF-α在颈椎后纵韧带骨化中的作用  

Expression of bFGF and TNF-α in the ossification of the posterior longitudinal ligament of the cervical spine

作　　者：王修文[1] 谯勇[1] 吴东进[1] 

机构地区：[1]山东大学第二医院骨科,山东济南250033

出　　处：《山东大学学报（医学版）》2007年第3期279-282,共4页Journal of Shandong University:Health Sciences

摘　　要：目的:探讨碱性成纤维细胞生长因子(bFGF)和肿瘤坏死因子(TNF-α)在颈椎后纵韧带中的表达水平及在骨化发病机制中的作用。方法:采用免疫组织化学法,对30例骨化颈椎后纵韧带标本进行bFGF和TNF-α检测,与40例正常颈椎后纵韧带标本进行对照,再利用SPSS10.0行FISHER精确检验,分析有无统计学意义。结果:正常对照组bFGF表达10例阳性,30例阴性,阳性率25%;颈椎后纵韧带骨化组bFGF表达24例阳性,6例阴性,阳性率80%,差异有统计学意义(P<0.05)。正常对照组TNF-α表达32例阳性,8例阴性,阳性率80%;颈椎后纵韧带骨化组TNF-α表达10例阳性,20例阴性,阳性率33.3%,差异有统计学意义(P<0.05)。结论:bFGF的异常高表达和TNF-α的低表达促进了颈椎后纵韧带骨化的发生,这两种因子的异常表达在该病发病机制中有重要作用。Objective： To investigate the expression and function of bFGF and TNF-α in the ossification of the posterior longitudinal ligament（OPLL） of the cervical spine. Methods： Thirty specimens and 40 normal controls were used in this study. The immunohistochemical method （SABC） was applied to determine the expression levels of bFGF and TNF-α. SPSS（version 10.0） software was used to analyze the data. Results： Ten specimens of normal posterior longitudinal ligaments were positive for bFGF （25%） and twenty-four specimens with COPLL were positive for bFGF （80%）. Thirty-two specimens of normal posterior longitudinal ligaments were positive for TNF-α（80% ） and ten specimens with COPLL were positive for TNF-α （33.3%）. There was a significant difference （P 〈 0.05）. Conclusions： The expression of bFGF in patients with COPLL is higher than that in normal postefor longitudinal ligaments（ P 〈 0,05）, High expression of bFGF and low expression of TNF-α play an important role in the pathogenesis of COPLL.

关 键 词：纵韧带 成纤维细胞生长因子2 肿瘤坏死因子 

分 类 号：R681.53[医药卫生—骨科学]