蝎毒多肽提取物对肿瘤生长和细胞免疫功能的影响  

Effect of polypeptide extract from scorpion venom on tumor growth and cellular immunity in rats with W256 sarcocarcinoma

作　　者：张维东[1] 张月英[1] 王朝霞[1] 王兆朋[1] 贾青[1] 

机构地区：[1]山东省医学科学院基础医学研究所山东现代医用药物与技术重点实验室,山东济南250062

出　　处：《山东大学学报（医学版）》2007年第3期286-289,共4页Journal of Shandong University:Health Sciences

基　　金：国家自然科学基金资助课题(30371841);山东省自然科学基金资助课题(2003C02)

摘　　要：目的:观察蝎毒多肽提取物(PESV)对大鼠W256癌肉瘤抑制作用及其对荷瘤大鼠细胞免疫功能的影响。方法:将30只Wistar荷瘤大鼠,随机分为模型组、5-FU治疗组和蝎毒治疗组,连续灌胃给蝎毒多肽提取物7 d,处死,计算各组抑瘤率;流式细胞术(FCM)检测外周血T淋巴细胞亚群变化;酶联免疫吸附试验(ELISA)法测定血清中细胞因子INF-γ和IL-4含量。结果:蝎毒治疗组抑瘤率为55.21%。与模型组相比较,蝎毒治疗组荷瘤大鼠外周血中CD4+T细胞比例明显提高,CD8+T细胞明显降低,CD4+T/CD8+T值明显增高,差异均具有统计学意义(P<0.05);血清中细胞因子INF-γ含量明显升高,而IL-4含量显著降低,差异均具有统计学意义(P<0.05)。结论:蝎毒多肽提取物对W256荷瘤大鼠肿瘤有明显抑制作用,通过提高细胞免疫功能,逆转荷瘤机体Th1/Th2漂移,改变荷瘤机体免疫抑制状态,发挥抗肿瘤作用。Objective： To study the inhibitory mechanism of polypeptide extract from scorpion venom （PESV） on W256 sareocareinoma and its influence on T subsets in rats. Methods： Tumor models were established by subcutaneously implanting W256 tumor cells into thirty Wistar rats. The tumor-bearing rats were randomly divided into three groups： the model group, the 5-fluorouracil（5-FU） group, and the PESV group. PESV was intragastrically subjected to the rats of the experimental group for 7 days. Then, the tumor-bearing rats were killed, and T subsets in the peripheral blood were determined by flow cytometry （FCM）. The presence of interferongamma （INF-γ） and interleukin-4 （IL-4） were screened by an enzyme-linked immunosorbent assay （ELISA）. Results： PESV had an inhibitory effect on tumor growth, and the growth inhibitory rate was 55.2 %. The number of CD4 ＋ T and the CD4 ＋ T/CD8 ＋ T ratio of W256-bearing rats treated with PESV significantly increased compared with the model group （P 〈 0.05）. The expression level of interferon-gamma （INF-γ） was up-regulated and of interleukin -4 （IL-4） down-regulated. Compared with the control group, the difference was significant（ P 〈 0.05）. Conclusion： PESV has an inhibitory effect on tumor growth by strengthening the immunocellular function. It could be regarded as a candidate of tumor inhibition.

关 键 词：蝎毒液类 免疫活性 CD4+T/CD8+T TH1/TH2漂移 

分 类 号：R979.1[医药卫生—药品]