饮水型砷暴露人群p16基因甲基化的研究  

A Study on Methylation of p16 Gene in Population Exposed to Arsenic via Drinking Water

作　　者：卢光明[1] 姚孝元[2] 张世英[3] 李振龙 蔡庆[5] 张文[5] 

机构地区：[1]中国人民解放军总医院门诊部,北京100853 [2]中国疾病预防控制中心环境与健康相关产品安全所 [3]内蒙古巴彦淖尔市卫生防疫站 [4]内蒙古杭锦后旗卫生防疫站 [5]空军总医院分子生物学中心

出　　处：《中国地方病防治》2007年第2期95-97,共3页Chinese Journal of Control of Endemic Diseases

基　　金：国家自然科学基金资助项目(39800123)

摘　　要：目的研究砷暴露人群p16基因甲基化发生情况。方法分别在饮水型砷中毒病区选取40例典型确诊地砷病患者、40名病区非患者及40名非病区的正常人(2个对照组),并进行年龄、性别匹配,采用甲基化特异性PCR技术测定人群血标本p16基因甲基化水平。结果地砷病患者及对照人群p16基因的甲基化率分别为65.0%、47.5%和20.0%;随着饮水砷浓度的增高,p16基因甲基化率也是逐渐增高。结论饮水砷暴露可引起人群p16基因甲基化发生率升高。Objective To study occurence of methylation of p16 gene in population of Arsenic exposure. Methods One Case -guoup was 40 typical and definitely diagnosed patients with endemic arsenism,selected from thd areas prevalent with it. Two control -groups were selected from the areas prevalent and non -prevalent with endemic arsenism,respectively, matched on age and sex. Methylation of p16 gene in the blood specimens were analyzed for all the subjects with MS - PCR techniques. Results The positive rates of p16 hypermethylaion of blood specimens in one case - group and two control - groups selected from the areas prevalent with endemic arsenism and its neighboring non - prevalent villages,were 65.0% ,47.5% and 20.0% ,respectively. Concurrently,the frequency of methylation was significantly increased with the increasing concentrations of arsenic in their drinking water in the areas prevalent with endemic arsenism. Conclusions Arsenic exposure through drinking water can induce higher frequency of methylation of p16 gene in the blood specimens of residents in the areas prevaltent with endemic arsenism.

关 键 词：地砷病 P16基因 病例对照研究 危险因素 LOGISTIC回归 

分 类 号：O613.63[理学—无机化学]