肝素化胶原/壳聚糖多孔支架的制备及其血管化的研究  

Study on Fabrication of Heparinized Collagen/chitosan Porous Scaffold and its Angiogenesis in vivo

作　　者：石海飞[1] 韩春茂[1] 毛峥伟[2] 陈轶欣[1] 马列[2] 高长有[2] 

机构地区：[1]浙江大学医学院附属第二医院烧伤科,杭州310009 [2]浙江大学高分子科学与工程学系,杭州310027

出　　处：《中国生物医学工程学报》2007年第2期296-302,共7页Chinese Journal of Biomedical Engineering

基　　金：国家自然科学基金资助项目(30371464);浙江省自然科学基金资助项目(302036)

摘　　要：本研究旨在构建一种能快速血管化的人工真皮替代物。用冻干法制备了胶原/壳聚糖多孔支架,并对其进行肝素化,观察此支架的结构特征、亲水性、体外降解性和组织相容性,同时将血管生成素引入到此支架,对复合有血管生成素的肝素化支架的体内血管化进行了初步研究。结果表明,肝素化胶原/壳聚糖多孔支架具有合适的三维多孔结构、良好的吸水性和较理想的酶解稳定性,体内实验表明,此支架具有良好的组织相容性,血管生成素可加快支架的血管化。To develop an artificial dermal substitute capable of receiving rapid angiogenesis. Collagen/chitosan porous scaffold was fabricated by freeze-drying method, and heparinized using N-（ 3-dimethylaminopropyl）-N＇ ethylcarbodiimide （EDC） and N-hydroxysuccinimide （NHS）. The morphology, hydrophilicity, in vitro biodegradation and tissue compatibility of the scaffold were investigated. After combined with angiogenin, the in vivo angiogenesis of the scaffold was studied as well. The results showed that with a suitable three-dimensional porous structure, the heparinized collagen/chitosan porous scaffold possessed good hydrophilicity and stability for collagenase degradation. In vivo study also demonstrated that the heparinized scaffold had good tissue compatibility, and its angiogenesis could be enhanced by angiogenin.

关 键 词：胶原/壳聚糖 支架 肝素化 血管生成素 血管化 

分 类 号：R318.08[医药卫生—生物医学工程]