绿僵菌侵染对椰心叶甲酚氧化酶活性的影响  

Effects of Metarhizium anisopliae on the activity of Phenoloxidase in Brontispa longissima

作　　者：王九辉[1] 张世清[1] 黄俊生[1] 

机构地区：[1]中国热带农业科学院环境与植物保护研究所热带作物生物技术国家重点实验室,海口571101

出　　处：《昆虫知识》2007年第2期252-255,共4页Entomological Knowledge

基　　金：科技部社会公益研究专项(2005DIB4J043)

摘　　要：对椰心叶甲Brontispa longissima(Gestro)成虫血淋巴中酚氧化酶的特性进行分析,并研究绿僵菌(Metarhizium anisopliae)侵染对血浆甲酚氧化酶活性的影响。结果显示,椰心叶甲成虫的血浆及血细胞裂解液中均检测到酚氧化酶活性,且昆布多糖及胰蛋白酶可显著提高其活性。绿僵菌MA-4侵染组在侵染后第1至第5d的血浆酚氧化酶活性高于未侵染组(P<0.05),但是椰心叶甲成虫体内注射10μg昆布多糖后,侵染组的酚氧化酶活性显著低于未侵染组(P<0.05),表明绿僵菌一方面对可激活椰心叶甲的酚氧化酶原激活系统,另一方面又可抑制昆布多糖对椰心叶甲酚氧化酶原激活系统的诱导作用。The activity of phenoloxidase in the haemolymph of Brontispa longissima （Gestro）infested by Metarhizium anisoplias MA-4 was investigated. The phenoloxidase activity in normol insects was found primarily on the plasma and hemocyte lysate solution, and increased significantly when laminarin and typsin as elicitors was present. The activity of phenoloxidase in infected group was higher than that in uninfected group （P 〈 0.05 ）, but the activity of phenoloxidase induced by injection 10 ttg laminarin in infected group is significant lower than that in uninfected group （P 〈 0.05 ）. These results suggested that M. anisopliae infection activated the prophenoloxidase system of B. longissima, and also suppress the prophenoloxidase system activation which induced by laminarin.

关 键 词：椰心叶甲 酚氧化酶 绿僵菌 血淋巴 

分 类 号：S476.1[农业科学—农业昆虫与害虫防治]