丹参酮Ⅱ-A磺酸钠对纤维化人肾间质成纤维细胞体外增殖及cyclin E蛋白表达的影响  

Effect of sodium tanshinone Ⅱ-A sulfonate on proliferation and cyclin E expression in human renal interstitial fibroblasts in vitro

作　　者：孙兴旺[1] 曹灵[2] 于国华[1] 唐学清[1] 郭庆喜[1] 张弦[1] 王巍[1] 许凯[1] 

机构地区：[1]泸州医学院基础医学院病理学教研室,泸州646000 [2]泸州医学院附属医院肾内科,泸州646000

出　　处：《第三军医大学学报》2007年第7期585-587,共3页Journal of Third Military Medical University

基　　金：四川省中医药管理局科研基金(200022)~~

摘　　要：目的研究丹参酮Ⅱ-A磺酸钠(sodium tanshinoneⅡ-A sulfonate,DS-201)对人肾间质纤维化来源的成纤维细胞(human renal interstitial fibroblasts,hRIFs)体外增殖及细胞周期素E(cyclin E)基因表达的影响,探讨该药治疗肾间质纤维化的作用机制。方法体外培养并鉴定hRIFs;用四甲基偶氮唑(MTT)法检测对照组与不同DS-201浓度组hRIFs的增殖活性;用免疫细胞化学S-P法和图像分析技术检测对照组与不同DS-201浓度组hRIFs cyclin E基因的表达。结果随药物浓度的升高和作用时间的延长,抑制率逐渐升高,抑制作用逐渐增强,呈剂量依赖性和时间依赖性。用药组阳性细胞的平均光密度值在第3、5、7、9天显著低于对照组(P<0.05,P<0.01),第1天用药各组与对照组之间无统计学差异(P>0.05)。结论①DS-201对hRIFs体外增殖有显著抑制作用,可能是其治疗肾间质纤维化的机制之一。②DS-201抑制hRIFs体外增殖可能是通过抑制细胞中cyclin E基因的表达,阻滞细胞通过G1/S关卡,延长细胞周期实现的。Objective To study the effect of sodium tanshinone Ⅱ-A sulfonate （ DS-201 ） on the proliferation of human renal interstitial fibroblasts （hRIFs） and the expression of cyclin E. Methods After hRIFs were cultured and identified, MTT was used to detect the cells at passage 3-4 in present or absent of DS-201at different concentrations. The expression of cyclin E was analyzed by immunocytochemistry and the image-analytical technique. Results With the increase of the drug concentration and prolongation of action time, the inhibitory ratio was increased in a dose- and time-dependent manner. The expression of cyclin E was stronger in drug groups than in control group on the 3rd, 5th, 7th and 9th day （P 〈 0.05,P 〈 0.01 ）. Conclusion DS-201 significantly inhibits the hRIFs in vitro,which may be one of its mechanisms to suppress renal interstitial fibrosis. The mechanism of this inhibition may be due to that sodium tanshinone Ⅱ-A sulfonate inhibits the expression of cyclin E, thus blocks the G1/S point and extents the cell cycle.

关 键 词：丹参酮Ⅱ-A磺酸钠 肾脏 纤维化 成纤维细胞 细胞周期素E 

分 类 号：R285.5[医药卫生—中药学] R329.28[医药卫生—中医学]