基于survivin的“模拟病毒”瘤苗的制备和鉴定  

Preparation and identification of mimovirus tumor vaccine based on survivin

作　　者：杨曌[1] 牛微[1] 姜曼[1] 吴玉章[1] 赵建平[1] 王莉[1] 

机构地区：[1]第三军医大学基础医学部全军免疫学研究所,重庆400038

出　　处：《第三军医大学学报》2007年第8期705-707,共3页Journal of Third Military Medical University

基　　金：国家自然科学基金(30400437;30371329)~~

摘　　要：目的制备、鉴定基于肿瘤抗原survivin的“模拟病毒”瘤苗。方法在特定的条件下将阳离子肽和DNA制备成模拟病毒颗粒样的结构,并以扫描电镜、DNA阻滞试验、DNaseⅠ保护试验和免疫印迹(Western blotting)对该疫苗进行制备鉴定。结果在NaCl浓度为87.5 mmol/L,电荷比为2的制备条件下,该疫苗能形成颗粒,并可有效介导瘤苗所携带基因的表达。结论该研究成功制备了基于肿瘤抗原survivin的“模拟病毒”瘤苗,为新型的肿瘤疫苗的设计奠定了基础。Objective To prepare and identify the mimovirus tumor vaccine based on survivin. Methods Under specific condition, peptide and DNA were condensed into like-virus particle, and the preparing parameters were optimized by assays of DNA gel retardation, DNase I protection and identified by Western blotting. Antigen presentation was also studied by RMA-S/Kd cells. Results The vaccine was condensed into homogeneous particles at charge ratio of 2 in 87.5 mmol/L NaCl concentration, which could effectively mediate expression of the genes carried by vaccine particles. Conclusion The mimovirus tumor vaccine based on survivin was prepared successfully, providing a good foundation for the design of new tumor vaccine.

关 键 词：基因转染 阳离子肽 疫苗制备 疫苗设计 

分 类 号：R392-33[医药卫生—免疫学] R392.11[医药卫生—基础医学]