对自制全氟化碳乳剂静脉输注安全性的实验研究  

Study on the safety of intravenous infusion with self-made perfluorocarbon emulsion in rats

作　　者：樊毫军[1] 刘书盈[2] 张健鹏[1] 刘又宁[3] 

机构地区：[1]武警总医院呼吸科,北京100039 [2]济南军区总医院呼吸科 [3]解放军总医院呼吸科

出　　处：《中国呼吸与危重监护杂志》2007年第2期123-126,I0002,共5页Chinese Journal of Respiratory and Critical Care Medicine

基　　金：国家"十五"重大攻关课题基金资助项目(编号:2003BA712A07-01);武警总医院科研课题资助项目(编号:WZ200527)

摘　　要：目的探讨自制全氟化碳乳剂(PFE)静脉输注的安全性。方法35只雄性Wistar大鼠随机分为对照组(5只)和PFE组(30只)。腹腔麻醉后,对照组静脉注射生理盐水10 mL/kg后2 h处死,PFE组大鼠于静脉注射PFE后5 min经眼眶静脉取血200μL测定全氟化碳(PFC)浓度,然后分别于2 h,4 h,6 h,24 h,48 h和10 d随机处死5只。观察各组血液PFC浓度,PaO2水平,GPT、GOT、BUN、Cr浓度,肺脏、肝脏、肾脏常规病理变化。结果PFE组大鼠活动、反应正常,无一例死亡;肝脏、肾脏病理无明显异常;PFC颗粒广泛分布于肺泡及肺微血管。PFE组2 h,4 h,6 h,24 h时间点GPT及GOT均显著高于对照组(P均<0.05);第10 d降至(92.5±6.4)U/mL和(163.9±1.3)U/mL,与对照组比较无明显差异。BUN、Cr水平与对照组比较无明显差异(P均>0.05)。静脉注射PFE10 mL/kg后5 min,2 h,4 h,6 h,24 h,48 h,10 d血中PFC浓度分别为(20.0±1.8)mg/mL,(1.8±0.7)mg/mL,(1.5±0.6)mg/mL,(1.2±0.4)mg/mL,(0.5±0.2)mg/mL,(0.2±0.03)mg/mL,0 mg/mL。PFE组2 h时间点PaO2显著高于对照组[(119.2±8.6)mm Hg比(99.6±4.7)mm Hg,P<0.05]。结论静脉输注PFE对大鼠一般情况、肺脏、肝脏、肾脏脏器病理无明显影响,具有较好的安全性。静脉输注PFE后PFC颗粒可广泛分布于肺泡及肺微血管,并显著提高健康大鼠PaO2,可试用于急性肺损伤的防治研究。Objective To assess the safety of intravenous infusion with sell-made perfluoroearben emulsion （PFE） in rots.Methods 35 Wistar rats were randomly divided into a normal control group and six PFE groups （ n = 5 in eaeh group）. The control group was injected intravenously with normal saline and six PFE groups with infused with PFE. Blood samples were obtained from the PFE groups 5 min after injection via vena orbitalis for perfluoroearben （PFC） measurement. The control group were sacrificed at 2 hours after injection, and the six PFE groups were killed at 2 h,4 h,6 h,24 h,48 h and 10 d after injection,respectively. The blood levels of PFC, PaO2, GPT, GOT, BUN and Cr were measured. Pathological changes of lung, liver and kidney were also observed. Results All mrs were in good health state after PFE intravenous injection with no obvious pathological changes in liver and kidney. PFC was wide distributed throughout the pulmonary alveoli and capillaries. The levels of GPT and GOT were higher in the PFC groups at 2,4,6 and 24 h than which in the control group （ all P 〈0.05） ,but there were no significant difference between the PFE 10 d group and the control group.The levels of BUN and Cr had no remarkably differences in all groups. Blood PFC concentrations were （20.0 ± 1.8）mg/mL, （ 1.8 ± 0.7） mg/mL, （ 1.5 ± 0.6） mg/mL, （ 1.2 ± 0.4） mg/mL, （0.5 ± 0.2） mg/mL, （0.2 ± 0.03）mg/mL,0 mg/mL in the PFE groups at 5 min,2 h,4 h,6 h,24 h,48 h,10 d after injection,respectively. PaO2 of the PFE 2 h group was significant higher than that in the eontrol group [ （119.2 ± 8.6）mm Hg vs（99.6±4.7）rran Hg, P 〈0.05.Conclusion Intravenous infusion with PFE （10 mL/kg） can enhance PaO2 without obvious pathological changes of lung,liver and kidney,implying a promising role in the treatment of acute lung injury.

关 键 词：全氟化碳 乳剂 静脉内输注 安全性 

分 类 号：R96[医药卫生—药理学]