妊娠、分娩以及泌乳期大鼠下丘脑中食欲素及其受体表达的变化  

CHANGES IN THE EXPRESSION OF OREXIN AND ITS RECEPTORS IN THE RAT HYPOTHALAMUS DURING PREGNANCY,PARTURITION AND LACTATION

作　　者：王俊波[1] 凌树才[1] 沈岚[2] 孙百强[1] 朱晞[1] 

机构地区：[1]浙江大学医学院解剖学教研室,杭州310058 [2]杭州市红十字会医院妇产科,杭州310004

出　　处：《解剖学报》2007年第2期134-138,共5页Acta Anatomica Sinica

基　　金：国家自然科学基金资助项目(30570586);浙江省教育厅科研项目(20061415)资助

摘　　要：目的观察大鼠下丘脑中食欲素(Orexin)及其受体(OX1R、OX2R)在妊娠、分娩以及泌乳期表达的变化,以探讨Orexin与生殖功能之间的关系。方法通过竞争性逆转录多聚酶链式反应(Competitive RT-PCR)和免疫组织化学方法测定大鼠下丘脑中Orexin前体(Prepro-OX)、Orexin-A、OX1R和OX2R在妊娠、分娩以及泌乳期的表达。结果Orexin-A和OX1R阳性神经元主要分别存在于大鼠下丘脑外侧区(LHA)以及妊娠和泌乳期大鼠的下丘脑室旁核(PVN)和视上核(SON)。泌乳第1d的Prepro-Orexin mRNA和OX1R的表达水平明显高于妊娠后期和泌乳期。OX2R的表达在生殖各时期之间没有明显改变。结论Orexin可能参与大鼠泌乳早期的生殖功能调节,下丘脑的PVN及SON可能是其重要的作用部位。Objective To investigate the possible role of orexin in reproduction and examine the changes in the expression of orexin and its receptors （OX1R, OX2R） in the rat hypothalamus during pregnancy, parturition and lactation. Methods The expressions of prepro-orexin （prepro-OX）, orexin-A, OX1R, and OX2R in the rat hypothalamus during pregnancy, parturition, and lactation were evaluated by competitive reverse transcription-polymerase chain reaction （RT-PCR） and immunohistochemistry assay. Results Orexin-A immunoreactive （ir） neurons and the OX1R subtype in neuronal cell bodies were mainly located in the lateral hypothalamic area （LHA） as well as in the magnocellular neurons of the paraventricular and supraoptic nuclei respectively in pregnant and lactating rats. The prepro-OX and OX1R mRNA levels on the 1st day of lactation were significantly higher than that during late pregnancy and lactation. No significant changes of OX2R expression were observed during the various reproductive phases. Conclusion Orexin might be involved in regulating reproductive function in early lactation through their binding sites in hypothalamic PVN and SON.

关 键 词：食欲素 食欲素受体 下丘脑 下丘脑外侧区 竞争性逆转录多聚酶链式反应 大鼠 

分 类 号：R338.2[医药卫生—人体生理学]