大鼠实验性脾气虚胃溃疡证病结合模型回肠5-HT及其受体和IL-2、IL-6变化的研究  

CHANGES OF 5-HT AND ITS RECEPTOR,IL-2,IL-6 IN ILEUM DURING EXPERIMENTAL SPLEEN-DEFICIENCY COMBINED WITH GASTRIC ULCER IN RATS

作　　者：赵素贤[1] 王秀琴[1] 杜鹃[1] 孙海梅[1] 曾晓蓓[1] 李宝红[1] 

机构地区：[1]首都医科大学组织学与胚胎学教研室,北京100069

出　　处：《解剖学报》2007年第2期226-230,共5页Acta Anatomica Sinica

基　　金：北京市中医药管理局科技发展基金资助项目(JJ-2003-11)

摘　　要：目的研究大鼠脾气虚胃溃疡证病结合模型中回肠5-羟色胺(5-HT)及其受体(5-HTR)、细胞因子IL-2、IL-6的变化,探讨脾虚胃溃疡的发病机制及加味四君子汤治疗脾虚证的机理。方法采用免疫组织化学技术、图像分析、放射免疫和免疫印迹法。结果大鼠脾气虚合并胃溃疡模型中5-HT阳性细胞主要分布于回肠黏膜上皮及肠腺细胞;5-HTR免疫反应阳性的细胞主要位于肠绒毛及腺体间结缔组织,在肠壁平滑肌、肌间神经丛也有表达;5-HT及其受体免疫反应阳性产物的含量均增加,免疫印迹法示回肠5-HTR含量增加,且随病程进展,而进一步升高;经过加味四君子汤治疗后,上述异常变化得到纠正。在造模初期,IL-2、IL-6的分泌活性增加,随着脾虚胃溃疡发病的进一步发展,IL-2、IL-6的分泌活性降低。结论大鼠脾气虚胃溃疡模型中回肠组织5-HT及其受体含量的增高,是大鼠脾虚胃溃疡发病的机理之一,加味四君子汤能通过纠正这些变化而发挥治疗作用。IL-2、IL-6分泌活性的改变可能与大鼠脾虚胃溃疡的病程发展相关。Objective To study the changes of 5-hydroxytryptamine（5-HT）,its receptor, IL-2 and IL-6 in rats＇ ileum with spleen deficiency combined with gastric ulcer. To explore the pathogenesis of this disease and the therapic mechanism of sijunzi decoction. Methods Immunohistochemical technique, image analysis, radioimmunoassay and Western blotting were used. Results The 5-HT-pesitive cells of rat ileum were detected in mucosal and glandular cells. The 5-HTR-positive cells of rat ileum were found in the connective tissues between the intestinal villus and intestinal glands, and in neuroplex and smooth muscle tissues. The expression of 5HT and 5-HTR in ileum increased during the experimental spleen deficiency combined with gastric ulcerin rats. The 5-HTR level of rat ileum was higher. After treatment with Sijunzi decoction, all the changes have been reversed. The level of IL-2 and IL-6 increased during the early period of this disease. When the disease doveloped, the synthesis or secretion of IL-2 and IL-6 decreased. Conclusion The increased contents of 5-HT and its receptor in ileum tissues might be one of the causes of spleen-deficiency combined with gastric ulcer in rats. The mechanism of Sijunzi decoction is to reverse the changes. And the changed activity of IL-2 and IL-6 may be related with the degree of the disease.

关 键 词：5-羟色胺 IL-2 IL-6 免疫组织化学 放射免疫测定 免疫印迹法 脾虚胃溃疡模型 大鼠 

分 类 号：R256.3[医药卫生—中医内科学]