灵芝孢子降低维甲酸诱导的孕鼠胚胎神经管畸形的发生  

GANODERMA SPORES REDUCING THE OCCURRENCE OF EMBRYONIC NEURAL TUBE DEFECTS INDUCED BY RETINOIC ACID IN PREGNANT MICE

作　　者：熊轶[1] 曾园山[1] 张伟[1] 陈穗君[1] 钟志强[1] 

机构地区：[1]中山大学中山医学院组织学胚胎学教研室神经科学研究室,广州市中山二路74号510080

出　　处：《解剖学报》2007年第2期231-237,共7页Acta Anatomica Sinica

摘　　要：目的探讨灵芝孢子能否促进受维甲酸诱导,而停滞在G0/G1期的胚胎神经管神经上皮细胞重新进入细胞周期循环,继续增殖和分化,减少神经管畸形的发生。方法于实验对照组和灵芝孢子组小鼠孕期胚胎(E)7.75d时一次性胃饲全反式维甲酸,造成这两组孕鼠的胚胎出现神经管畸形。然后,给予灵芝孢子组孕鼠胃饲灵芝孢子溶液。在孕期E10.5d时取出两组孕鼠的胚胎,分别计数胚胎的神经管畸形发生率。应用免疫荧光组织化学染色和流式细胞仪检测胚胎神经管神经上皮的巢蛋白(nestin)表达情况。应用DNA定量荧光染色和RT-PCR技术检测胚胎神经管依赖细胞周期蛋白激酶2(Cdk2)mRNA和Cdk4 mRNA的转录。结果灵芝孢子组孕鼠胚胎的神经管畸形发生率显著低于实验对照组。神经管神经上皮细胞表达nestin的百分率也明显大于实验对照组。实验对照组孕鼠胚胎的神经管细胞在G0/G1期的比例则显著高于灵芝孢子组,但是实验对照组神经管细胞S期的比例低,低于灵芝孢子组形态正常的胚胎神经管细胞。RT-PCR检测发现,灵芝孢子组孕鼠胚胎神经管细胞能够正常转录Cdk4 mRNA,而实验对照组则低转录Cdk4 mRNA。结论灵芝孢子能够减少维甲酸诱导的妊娠小鼠孕期E10.5d胚胎神经管畸形的发生。Objective To explore whether ganoderma spores could help the neural epithelial cell of embryonic neural tube stagnated by retinoic acid in G0/G1 phase reenter the cell cycle, keep on proliferating and differentiating, and could reduce the occurrence of neural tube defects （NTDs）. Methods When E7.75d, mice of the control group and the ganoderma spores group were given intragastrically onetime retinoic acid all-trans. Then the ganoderma spores solution was given intragastrically to the ganoderma spores group. At E10.5d, The embryos of the two groups were taken out and the ratio of NTDs was counted respectively histochemistry and flow cytometry were applied to examine the expression of nestin, and DNA quantity staining and RT-PCR to detect the mRNA transcriptions of Cdk2 and Cdk4. Results The radio of NTDs in the ganoderma spores group was lower than that in the control group remarkably, but the level of nestin was higher. With the neural tube cell cycles compared the cell radio of G0/G1 phase in the control group was higher than that in the ganoderma spores group, while the ratio in the S phase in the control group, it was lower than that of the normal embryos in the ganoderma spores group. The neural epithelial cell of embryonic neural tube in the ganoderma spores group could transcript Cdk4 mRNA normally, with a low transcription rate in the control group. Conclusion Ganoderma spores can reduce the occurrence of embryonic NTDs induced by retinoic acid in pregnant mice.

关 键 词：灵芝孢子 维甲酸 神经管畸形 巢蛋白 依赖细胞周期蛋白激酶 胚胎 小鼠 

分 类 号：R338.2[医药卫生—人体生理学]