黑龙江地区肾综合征出血热患者中病毒基因检测及流行病学意义  

Study on the Hantavirus gene detection from peripheral blood of patients with HFRS in Heilongjiang province and it＇s epidemiological significance

作　　者：陈焕永[1] 曾令兰[3] 张新[1] 姜宏齐[1] 邵风娟[1] 李庆刚[1] 卢宝玲[1] 李琳[2] 

机构地区：[1]哈尔滨医科大学第一临床医学院传染科,150001 [2]哈尔滨医科大学第一临床医学院检验科,150001 [3]华中科技大学同济医学院附属协和医院传染科

出　　处：《中华微生物学和免疫学杂志》2007年第3期282-284,共3页Chinese Journal of Microbiology and Immunology

基　　金：黑龙江省科学技术厅攻关课题（GC030609）

摘　　要：目的进一步了解黑龙江地区HFRS患者中汉坦病毒的基因类别，同时对该病近年来的流行病学特征进行初步分析。方法采用RT-PCR技术对31份肾综合征出血热（mRS）患者早期血清中的病毒进行基因分型，并根据患者发病季节分成两个感染时段，即冬季（2003年11月—2004年2月），春夏季（2004年4月-2004年9月），再结合临床症状综合分析。结果31份血清中，共有22份血清检测阳性，其中冬季感染时段14份血清，有8例检出阳性（Ⅰ型5例，Ⅱ型3例）；春夏季感染时段17份血清，有14例检出阳性（Ⅰ型5例，Ⅱ型9例）。结论黑龙江地区不仅存在汉坦病毒基因Ⅰ型，且同时存在Ⅱ型；冬季以Ⅰ型为主，春夏季以Ⅱ型为主。Objective To further understand the Hantavirus genotype from peripheral blood of patients with HFRS and study the epidemiological significance of this disease in Heilongjiang province in recent years. Medthods Thirty-one serum samples of clinically diagnosed patients with HFRS were examined by RT-PCR to decided the genetic subtype. On the basis of the incidence of patients infected season, we divide it into two slots： winter （Nov, 2003-Feb, 2004）; spring and sununer （April, 2004-Sep, 2004）, further analysis made with clinical symptoms. Results There are twenty-two sera identified as positive among thirty-one sera. Fourteen sera in winter were detected, among the total, eight are identified as positive （type Ⅰ ： five cases, type Ⅱ ： three） ; Seventeen serum were detected in spring and summner, fourteen are identified as positive （type Ⅰ ： five cases, type Ⅱ ： nine）. Conclusion The Hantavirus strains of type Ⅰ and Ⅱ are both exist in Heilongjiang. In winter, mainly type Ⅰ existed, while type Ⅱ mainly existed in spring and summer.

关 键 词：汉坦病毒 肾综合征出血热 流行病学 基因分型 

分 类 号：R686[医药卫生—骨科学]