田胡止痛颗粒对大鼠椎间盘磷脂酶A_2及神经根炎性细胞的影响  

Effects of Tianhu acesodyne grains (田胡止痛颗粒) on the activity of phospholipase A_2 of intervertebral disc and inflammatory cells of nerve root of rats

作　　者：翁凤泉 康乐 黄经光 陈日荣 周美容 翁志辉 欧阳厚淦[2] 

机构地区：[1]江门市五邑中医院,广东江门529031 [2]湖南中医药大学

出　　处：《中国骨伤》2007年第3期176-179,共4页China Journal of Orthopaedics and Traumatology

基　　金：广东省中医药管理局课题(编号:1050123)

摘　　要：目的:应用自体椎间盘移植的动物模型探讨田胡止痛颗粒治疗腰椎间盘突出症的相关机制。方法:对72只大鼠随机分为假手术组(SG)、模型对照组(CG)、芬必得组(FG)、田胡止痛颗粒组(TG),每组18只,采用自体尾椎椎间盘植入L5神经根背侧的方法制备大鼠腰椎间盘突出的非压迫性动物模型。各组大鼠于术后1 d开始灌胃,SG和CG用蒸馏水,FG和TG分别用芬必得和田胡止痛颗粒的悬浊液,并分别于术后的7、14、28 d处死动物,检测原移植椎间盘(SG取残留尾椎的椎间盘组织)磷脂酶A2的活性,对L5神经根进行病理切片检查和炎性细胞计数。结果:TG椎间盘磷脂酶A2的活性在用药7 d后与CG相比有统计学差异(P<0.05),14及28 d后比较有统计学差异(P<0.01)。TG神经根的炎性细胞浸润数目在各时间段亦少于CG(P<0.05或P<0.01),神经根的损伤情况明显轻于CG。结论:田胡止痛颗粒能够降低大鼠突出椎间盘磷脂酶A2的活性,减轻神经根的炎性反应,促进神经功能的恢复。Objective： To explore the correlative mechanism of Tianhu acesodyne grains （ 田胡止痛颗粒）for the treatment of lumbar disc herniation（LDH） by using animal models which suffered from grafting operation of self intervertebral disc. Methods： Seventy-two rats were divided into 4 groups randomly：sham surgery groups （SG） ,model contrast group（CG） , Fenbid group（FG） ,Tianhu acesodyne grains（田胡止痛颗粒）（TG）. There were 18 rats in each group. The coecygeal intervertebral disc was transplanted to the dorsal part of the right 5th lumbar nerve root of rats to establish the non-compressive model of LDH. After one clay,the rats in SG and CG were fed with distilled water,while rats in FG were fed with Fenbid and rats in TG were fed with suspending liquid of Tianhu acesodyne grains（田胡止痛颗粒）. Each 6 rats of every group were chosen randomly and killed at 7th, 14th, 28th clays after operation. The transplanted intervertebral disc and the remnant coccygeal disc of SG were taken out and stored under 0 ℃. The activities of PLA2 were tested through method of trace acid titration. At the same time, the right nerve roots of 5th lumbar were observed by pathological section and the inflammatory cells were counted under optical microscope. Results： At 7th day after the drugs were given to the rats, the activities of PLA2 had significant difference in rats between TG and CG （P 〈 0. 05 ）, and much significant difference （P 〈 0. 01 ） at 14th and 28th days. The amount of inflammatory cells of TG was obviously smaller than that of CG（ P 〈 0.05 or P 〈 0.01 ）, and the damage of nerve roots of TG was much lighter than that of CG. Conclusion： Tianhu acesodyne grains （ 田胡止痛颗粒） can decrease the activity of PLA2 in prolapsed intervertebral disc of rats, alleviate the inflammatory reaction of nerve root and promote the function recovery.

关 键 词：腰椎 椎间盘移位 中药药理学 磷脂酶A 

分 类 号：R681.53[医药卫生—骨科学]