Purification and Structural Analysis of a Selective Toxin Fraction Produced by the Plant Pathogen Setosphaeria turcica  

作　　者：ZHANG Li-hui DONG Jin-gao WANG Chao-hua LI Zheng-ping 

机构地区：[1]Mycotoxin Laboratory, Agricultural University of Hebei, Baoding 071001, P.R.China [2]College of Chemistry, Hebei University, Baoding 071000, P.R.China

出　　处：《Agricultural Sciences in China》2007年第4期452-457,共6页中国农业科学（英文版）

基　　金：supported by National Excellent Teacher's Plan,Ministry of Education,China and Natural Science Foundation of Hebei Province,China(302318).

摘　　要：Thirteen fractions from the pathogenic plant fungus Setosphaeria turcica race 1 were separated and collected using high performance liquid chromatography （HPLC）. Their toxic activities were assayed through leaf puncturing on corn differentials （OH43, OH43Ht1, OH43Ht2, and OH43HtN）, and the results revealed that eight fractions were toxic and fraction 6 was specifically toxic to OH43Ht1, which could be taken as a gene-selective toxin fraction. Fraction 6 was finely purified via HPLC and condensed by freeze desiccation. Its chemical structure was analyzed with EI-MS, IR, HMBC, ^1H-NMR, and two-dimensional NMR. The results suggested that fraction 6 contained an unsaturated double bond, carbonyl and methylene groups with molecular weight of 142.Thirteen fractions from the pathogenic plant fungus Setosphaeria turcica race 1 were separated and collected using high performance liquid chromatography （HPLC）. Their toxic activities were assayed through leaf puncturing on corn differentials （OH43, OH43Ht1, OH43Ht2, and OH43HtN）, and the results revealed that eight fractions were toxic and fraction 6 was specifically toxic to OH43Ht1, which could be taken as a gene-selective toxin fraction. Fraction 6 was finely purified via HPLC and condensed by freeze desiccation. Its chemical structure was analyzed with EI-MS, IR, HMBC, ^1H-NMR, and two-dimensional NMR. The results suggested that fraction 6 contained an unsaturated double bond, carbonyl and methylene groups with molecular weight of 142.

关 键 词：Setosphaeria turcica gene-specific toxin fraction structure analysis high performance liquid chromatography （HPLC） 

分 类 号：S435.131.49[农业科学—农业昆虫与害虫防治] S432.44[农业科学—植物保护]