甜菜花叶病毒新疆分离物基因组3′末端序列分析  

Sequence analysis of 3' terminal region of genome of Beet mosaic virus Xinjiang isolate

作　　者：向海英[1] 李菁博[1] 王颖[1] 韩成贵[1] 李大伟[1] 于嘉林[1] 

机构地区：[1]中国农业大学植物病理学系农业生物技术国家重点实验室,北京100094

出　　处：《植物病理学报》2007年第2期204-206,共3页Acta Phytopathologica Sinica

基　　金：国家自然科学基金资助项目(30471136)

摘　　要：The 3’ terminal genomic 1 611 nucleotides of Beet mosaic virus Xinjiang isolate(BtMV-XJ)from China was determined(GenBank accession number DQ345522).The sequence started within a single open reading frame which was expected to encode part C-terminus of NIb protein of 201 amino acids,complete capsid protein(CP)of 276 amino acids and followed by a 3’ untranslated region(UTR)of 168 nucleotides.The comparison of sequence diversity among BtMV-XJ and other three previously reported isolates revealed that it shared 98.3%,98.1%,91.4% nucleotide identity,and 99.79%,99.58%,97.69% deduced aa sequence identity with the corresponding 3’ terminal regions of BtMV-SL,BtMV-UK and BtMV-US,respectively,and that most nucleotide mutations were silent with no effect on aa sequence.In addition,a virus-specific and ra-pid RT-PCR detection method of BtMV was also developed based on the nucleotide sequence obtained.The 3＇ terminal genomic 1 611 nucleotides of Beet mosaic virus Xinjiang isolate （BtMV-XJ） from China was determined （GenBank accession number DQ345522）. The sequence started within a single open reading frame which was expected to encode part C-terminus of NIb protein of 201 amino acids, complete capsid protein （CP） of 276 amino acids and followed by a 3＇ untranslated region （UTR） of 168 nucleotides. The comparison of sequence diversity among BtMV-XJ and other three previously reported isolates revealed that it shared 98.3%, 98.1%, 91.4% nucleotide identity, and 99.79%, 99.58%, 97.69% deduced aa sequence identity with the corresponding 3＇ terminal regions of BtMV-SL, BtMV-UK and BtMV-US, respectively, and that most nucleotide mutations were silent with no effect on aa sequence. In addition, a virus-specific and rapid RT-PCR detection method of BtMV was also developed based on the nucleotide sequence obtained.

关 键 词：花叶病毒 序列分析 分离物 甜菜 新疆 马铃薯Y病毒属 基因组 分子结构特征 

分 类 号：S435.72[农业科学—农业昆虫与害虫防治]