马铃薯S病毒的分子鉴定与检测  

Occurrence and molecular detection of Potato virus S (PVS) in Zhejiang province

作　　者：郎秋蕾[1] 陈集双[1] 傅天珍[1] 杜志游[1] 

机构地区：[1]浙江大学生命科学学院

出　　处：《植物病理学报》2007年第2期217-220,共4页Acta Phytopathologica Sinica

摘　　要：An isolate of PVS,HZ00P1,was obtained from nature infected Solanum tuberosum from Hangzhou,Zhejiang province.It was primarily identified by DAS-ELISA and host reaction tests.3’ end partial sequence of the virus isolate was cloned.Nucleic acid sequence of the cp gene and deduced cp amino acid sequence alignments were compared with 16 other isolates registered in GenBank.The results showed that the 17 PVS isolates were divided into two groups.Among them,two Andean isolates were classified to groupⅡ,HZ00P1 and the other 14 isolates were assigned to groupⅠ.Compared with other PVS isolates in group Ⅰ,PVS HZ00P1 had 93.1%-98.1% and 95.9%-99.3% homologies of nucleotide sequence and deduced amino acid sequence respectively,whereas its similarities to the groupⅡwere 81.4%-81.7% and 93.5%-93.9%.A survey of PVS occurrence in Zhejiang province was achieved by RNA spot hybridization(RSH).Among the 30 samples collected from different areas of the province,26.7% were found to have the infection of PVS.It is concluded that PVS has become a common virus in Zhejiang province.An isolate of PVS, HZ00P1, was obtained from nature infected Solanum tuberosum from Hangzhou, Zhejiang province. It was primarily identified by DAS-ELISA and host reaction tests. 3＇ end partial sequence of the virus isolate was cloned. Nucleic acid sequence of the cp gene and deduced cp amino acid sequence alignments were compared with 16 other isolates registered in GenBank. The results showed that the 17 PVS isolates were divided into two groups. Among them, two Andean isolates were classified to group Ⅱ, HZ00P1 and the other 14 isolates were assigned to group Ⅰ . Compared with other PVS isolates in group Ⅰ , PVS HZ00P1 had 93.1%-98.1% and 95.9%-99.3% homologies of nucleotide sequence and deduced amino acid sequence respectively, whereas its similarities to the group Ⅱ were 81.4%-81.7% and 93.5%-93.9%. A survey of PVS occurrence in Zhejiang province was achieved by RNA spot hybridization （RSH）. Among the 30 samples collected from different areas of the province, 26.7% were found to have the infection of PVS. It is concluded that PVS has become a common virus in Zhejiang province.

关 键 词：马铃薯S病毒 分子鉴定 检测 株系分化 PVS 潜隐病毒 麝香石竹 蚜虫传播 

分 类 号：S532[农业科学—作物学]