胭脂鱼弹状病毒糖蛋白基因在原核细胞中的表达  

Expression of Glycoprotein Gene of Chinese Sucker Rhabdovirus (CSRV) in E.coli

作　　者：许禔森[1] 

机构地区：[1]德州学院生物系,山东德州253023

出　　处：《德州学院学报》2007年第2期29-31,共3页Journal of Dezhou University

摘　　要：克隆、构建弹状胭脂鱼弹状病毒糖蛋白基因重组质粒,并进行表达和鉴定.用RT-PCR方法扩增出大小为1.5 kb的CSRV-G基因片断,构建重组克隆质粒pRSET-C/CSRV-G,并将其转化进入大肠杆菌DH5α原核表达系统,酶切鉴定证明重组质粒的正确性.经IPTG诱导后表达重组蛋白,利用SDS-PAGE对表达产物进行分析鉴定,诱导后能够表达预期的分子量为55 kD的融合蛋白.An about 1.5kb functional domain sequence of CSRV-G gene was obtained by using RT-PCR amplification. The amplified fragment was cloned into prokaryotic expression system pRSET-C vector and then was transformed into CaC12 treated E. coli DH5α competent cells respectively. Recombinants were detected with restriction enzyme digestion and further confirmed the interest insert by sequencing pRSET -C/CSRV-G plasmid, in the proper orientation. SDS-PAGE revealed that the highly expressed fusion protein is produced by inducing with IPTG, and it s molecular weight is around 55kD, which is the right size corresponding to the predicted value.

关 键 词：弹状胭脂鱼弹状病毒 基因克隆 基因表达 

分 类 号：Q785[生物学—分子生物学]