高效液相色谱法测定康宁颗粒中野黄芩苷的含量  

Determination of scutellarin in Kangning granula by high performance liquid chromatography

作　　者：陈璇[1] 薛瑞民[2] 白小红[1] 杨雪[1] 王晓园[1] 朱颖[1] 冯婷[1] 

机构地区：[1]山西医科大学药学院药物分析教研室,太原030001 [2]太原市中心医院药剂科

出　　处：《山西医科大学学报》2007年第4期320-323,共4页Journal of Shanxi Medical University

摘　　要：目的建立康宁颗粒中野黄芩苷的含量测定方法。方法采用高效液相色谱法C18柱,流动相为甲醇-水-醋酸(35∶61∶4);检测波长335nm。结果野黄芩苷在6.0-30.0μg/ml范围内与峰面积呈良好线性关系,回归方程Y=28437X+3224.4,r=0.9994。平均回收率为(98.8±1.4)%,日间和日内精密度均小于1.4%。结论采用高效液相色谱法作为康宁颗粒中野黄芩苷的含量测定方法,其操作简便、快速、准确可靠。Objective To develop a method to determine the content of scutellarin in Kangning granula. Method The high performance liquid chromatometry was used with the C18 column as static phase and the methanol-aquafer-acetic acid（35 ： 61：4）as the mobile phase at the violet detectable wavelength 335 nm. Results The linear range for scutellarin was 6.0 - 30.0 μg/ml, and the regression equation was Y = 28 437X ＋ 3 224.4, r ： 0. 999 4. The average recovery was （98.8 ＋ 1.4） %. The RSD of inter-day and intra-day were lower than 1.4%. Conclusion The high performance liquid chromatometry is fast, simple and accurate to determine the content of scutellarin in Kangning granula.

关 键 词：色谱法 高压液相 野黄芩苷 含量测定 

分 类 号：R927.2[医药卫生—药学]