食管癌易感性与STK15基因Phe31Ile多态性的关联研究  

Study on the association between STK15 Phe31Ile polymorphisms and esophageal squamous cell carcinoma

作　　者：王娜[1] 王贵英[2] 郭炜[1] 董秀娟[1] 李琰[1] 

机构地区：[1]河北医科大学第四医院分子生物学室,石家庄050011 [2]河北医科大学第四医院外二科,石家庄050011

出　　处：《中华流行病学杂志》2007年第4期394-397,共4页Chinese Journal of Epidemiology

基　　金：河北省普通高等学校强势特色学科资助项目

摘　　要：目的研究STK15基因Phe31Ile（91T→A）单核苷酸多态性（SNP）与河北省涉县人群食管鳞状细胞癌（ESCC）发病易感性的关系。方法采用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）方法检测了涉县296例ESCC患者和302例健康对照STK15 Phe31Ile（91T→A）SNP的基因型。结果吸烟及上消化道肿瘤（UGIC）阳性家族史均能显著增加ESCC的患病风险（OR值分别为1．68和1．77,95%CI分别为1．34～2．10和1．44～2．19）。STK15 Phe31Ile三种基因型（Phe/Phe、Phe/Ile、Ile/Ile）频率在ESCC患者组中分别为11．5%、34．8%和53．7%,与健康对照组（11．9%、36．8%和51．3%）相比差异无统计学意义（X^2=0．35,P=0．84）。以Phe/Phe基因型作参照,Phe/Ile基因型及IIe/Ile基因型分别与其相比,均未增加ESCC的发病风险（OR值分别为0．98和1．09,95%CI分别为0．57～1．69和0．65～1．82）。根据性别、吸烟状况及上消化道肿瘤（UGIC）家族史进行分层分析,也未发现病例组与对照组之间的差异有统计学意义。结论STK15基因Phe31Ile（91T→A）SNP可能与河北涉县人群ESCC的患病风险无关。Objective To study the relation between single nucleotide polymorphism （SNP） at the 91T→A（Phe31 Ile） position of the STK15 gene and the susceptibility of esophageal squamous cell carcinoma （ESCC） in She county -- a ESCC high incidence region in North China. Methods Polymerase-chain reaction（ PCR）-restriction fragment length polymorphism（RFLP） analysis was used to detect the genotypes of STK15 Phe31 Ile（91T→A） SNP, and the samples came from 296 ESCC patients and 302 healthy controls. Results The risk of ESCC significantly increased in the group which had been smoking or having a family history of upper gastrointestinal cancer（UGIC） （the OR = 1.68 and 1.77,95 % CI ： 1.34-2.10 and 1.44-2.19, respectively）. Rates of the three genotypes（Phe/Phe, Phe/Ile, Ile/Ile） of the STK15 Phe31 lie （91T→A） SNPs in ESCC patients were 11.5%, 34.8% and 53.7%, respectively, and were not significantly different from that in the healthy group（ 11.9 %, 36.8 % and 51.3 % ） （X^2 = 0.35, P = 0.84）. When compared to Phe/Phe genotype, Phe/Ile and lie/lie of STK15 91T→A（Phe31 Ile） did not show effect oil the risk of ESCC according to the odds ratio results which were 0.98 （95 % CI：O. 57-1.69） and 1.09 （0.65-1.82） respectively. STK15 91T→A （Phe31 Ile） SNP also did not significantly influence on the development of ESCC even the samples were stratified by sex, smoking status and family history of upper gastrointestinal cancer. Conclusion The STK15 Phe31 lie （91T→A） polymorphisms seemed irrelevant with the risk of ESCC in She county.

关 键 词：食管鳞状细胞癌 发病易感性 STK15基因 单核苷酸多态性 

分 类 号：R686[医药卫生—骨科学]