单细胞电泳技术检测低温保存猪精子的DNA损伤  

Effect of Cryopreservation on the DNA Damage of Boar Sperm with Single Cell Gel Electrophoresis

作　　者：李文烨[1] 李青旺[1] 江忠良[1] 张树山[1] 

机构地区：[1]西北农林科技大学动物科技学院,陕西杨凌712100

出　　处：《安徽农业科学》2007年第9期2581-2582,2618,共3页Journal of Anhui Agricultural Sciences

基　　金：河北省秦皇岛市科技局转基因动物生产基因工程药物研究项目(D08)

摘　　要：应用单细胞凝胶电泳技术（SCGE），对冷冻解冻后猪精子DNA的损伤情况进行研究。结果表明，冷冻解冻会对猪精子DNA造成损伤，其彗星率与鲜精相比差异显著（P〈0．05）；各组冻精之间，甘油添加量为2％～3％（v／v）时，冷冻对猪精子DNA造成的损伤最低，彗星率均低于其余各组（P〈0．05）。Boar sperm cryopreservation is an important method for germplasm conservation. However, the method may cause the change of DNA structure and component. The change of DNA structure may damage the boar sperm quality and result in defects in its descendant. Therefore,how to keep the sperm intact and how to avoid the damage caused by cryopreservation become an important issue. Thus, the effect of cryopreservation on the DNA damage of boar sperm was studied and the method of single cell gel electrophoresis（SCGE） was a simple and valid method to examine the damage of the sperms. And the degree of the DNA damage was estimated with the comet tail pattern formed by the DNA nuclei movement in the electricity field. SCGE was used to study the DNA damage of cryopreservation boar sperm. The result indicated that cryopreservation can cause boar sperm DNA damage and compared with the fresh sperm it had remarkable difference（ P 〈0.05）, the frozening caused lowest DNA damage than others when the glycerol with the concentration of 2% to 3% was suppled in experiment group（ P 〈0.05）.

关 键 词：猪精子 单细胞电泳 DNA损伤 甘油 

分 类 号：S814.1[农业科学—畜牧学]