汞离子对草鱼血清和肾脏溶菌酶活性的影响  

Effect of Hg^(2+) on Lysozyme Activity in Serum and Kidney of Grass Carp

作　　者：刘占才[1] 牛俊英[1] 孔祥会[2] 郭彦玲[2] 郭春丽[2] 

机构地区：[1]焦作师范高等专科学校生物系,河南焦作454001 [2]河南师范大学生命科学学院,河南新乡453007

出　　处：《安徽农业科学》2007年第9期2615-2616,共2页Journal of Anhui Agricultural Sciences

基　　金：河南省科技攻关项目(0624030022);河南省动物学重点学科资助项目

摘　　要：测定暴露于不同汞离子质量浓度(0、0.05、0.10、0.15、0.20、0.25 mg/L)下草鱼血清和肾脏溶菌酶(LSZ)活性。在试验剂量和时间范围内结果显示,汞对草鱼血清和肾脏LSZ活性有剂量—效应关系和Hormensis现象。在0.05和0.10 mg/L浓度胁迫下,分别对血清和肾脏LSZ有激活作用;在高于0.05和0.15 mg/L浓度胁迫下分别对血清和肾脏LSZ有显著的抑制作用(P<0.01)。试验表明汞离子对草鱼具有显著的免疫毒性。In this study, the biochemistry methods were adopted to determine the activity of Lysezyme （LSZ） in serum and kidney of Grass carp （ Ctenopharyngodon idella）exposed at different Hg^2 ＋ concentrations for 3-week. The results showed that with in a range of exposure dosages and duration, there existed linearity relationship between dosage-response in term of Hg^2＋ on LSZ in serum and kidney. LSZ activity in serum was significantly increased at 0.05 mg/L （P 〈0.05） and in kidney significantly increased at 0.10 mg/L （ P 〈0.01 ） .While LSZ activity in serum was markedly descended at more than 0.05 mg/L （P 〈0.01）,and in kidney markedly decreased at 0.20 mg/L and 0.25 mg/L（P 〈0.05 and P 〈0.01 ） .All the results indicated that Hg^2＋ possessed obviously immuno-toxicity to Grass carp.

关 键 词：HG^2+ 草鱼 溶菌酶 血清 肾脏 

分 类 号：S917[农业科学—水产科学]