香姜颗粒挥发油的萃取工艺研究  

Study on Extraction Technique for Xiangjiang Granula Essential Oil

作　　者：黎海妮[1] 王四旺[1] 王剑波[1] 常瑛[1] 涂剑波[1] 戚志华[1] 

机构地区：[1]第四军医大学药物研究所,西安市710033

出　　处：《中国药房》2007年第12期898-900,共3页China Pharmacy

摘　　要：目的：研究香姜颗粒挥发油超临界CO2流体萃取的最佳工艺.方法：以蒿本内酯含量和收油率为指标,95%乙醇为夹带剂,用正交试验法进行优选;用高效液相色谱法对蒿本内酯的含量进行测定,色谱柱为Phenomenex Luna C18（250mm×4.6mm,5μm）,流动相为甲醇-0.5%醋酸（30∶70）,检测波长为280nm.结果：香姜颗粒挥发油萃取的最佳工艺条件为萃取温度50℃,萃取压力45MPa,萃取时间3h,95%乙醇作为夹带剂.蒿本内酯在5.1～25.5 μg·mL-1浓度范围内线性关系良好（r=0.999 8）.结论：该工艺制备简便,操作可行,可为大生产提供理论依据.OBJECTIVE： To study the optimum supercritical- fluid extraction technique for Xiangjiang Granula essential oil. METHODS： The orthogonal test was adopted to optimize the extraction process using the content of Ligustilide and the yield rate of essential oil as indicators, and 95% ethanol as co-solvents. The content of Ligustilide was determined by HPLC, using Phenomenex Luna C18（250nm × 4.6nm, 5μm） as column and methanol - 0.5% glacial acetic acid（30 ： 70） as mobile phase, with the detection wavelength set at 280 nm. RESULTS： The optimal extraction conditions were： temperature at 50℃, pressure at 45MPa, extraction time for 3h, and 95% ethanol as co-solvents.The Ligustilide had a good linearty relationship between 5.1-25.5μg · mL^-1（r = 0.999 8） .CONCLUSIONS： This technique is easy, convenient and workable, and can provide theoretical support for production.

关 键 词：香姜颗粒 挥发油 蒿本内酯 超临界CO2流体萃取 正交试验 

分 类 号：R283[医药卫生—中药学]