银连口服液质量标准研究  

Study on Quality Standards for Yinlian Oral Solution

作　　者：漏德宝 陈蔚琳[1] 

机构地区：[1]上海市第八人民医院,上海市200235

出　　处：《中国药房》2007年第12期919-921,共3页China Pharmacy

摘　　要：目的：建立银连口服液的质量标准.方法：以薄层色谱（TLC）法鉴别方中黄芩、黄连、连翘;以高效液相色谱（HPLC）法测定黄芩苷的含量：色谱柱为Nova-Pak C18（150mm×4.6mm,4μm）,流动相为甲醇-0.3%磷酸溶液（40∶60）,检测波长为276nm,流速为1.0mL·min^-1.结果：TCL色谱中能明显检出黄芩、黄连、连翘.黄芩苷的检测浓度线性范围为1.68～16.8 μg·mL^-1（r=0.9998）;平均加样回收率为99.06%（RSD=0.79%）.结论：所建标准准确、可靠,可用于控制银连口服液的质量.OBJECTIVE： To establish the quality standards for Yinlian oral solution. METHODS： TLC was adopted to identify Radix Scutellariae, Rhizoma Coptidis, Fructus Forsythiae in Yinlian oral solution. HPLC was applied to determine the content of Baicalin in Yinlian oral solution, using Nova - Pak C18（150mm × 4.6mm, 4μm） as column, the mixture of methanol-0.3%phosphoric acid （40 ： 60） as mobile phase, with flow rate set at 1.0mL · min^-1 and detection wavelength at 276 nm. RESULTS： Radix Scutellariae, Rhizoma Coptidis and Fructus Forsythiae were clearly detected with TLC. The content of baicalin showed a good linear relationship at a range of 1.68-16.8μg · mL^-1（ r = 0.999 8） .The average recovery was 99.06% （RSD = 0.79% ）. CONCLUSIONS： The standard is accurate, reliable, and can be used for quality control of Yinlian oral solution.

关 键 词：银连口服液 黄芩苷 薄层色谱法 高效液相色谱法 

分 类 号：R927.1[医药卫生—药学] R286