毛细管电色谱聚丙烯酰胺类整体柱对芳香酮类化合物分离性能考察  

Evaluation of Separation Performance of Aromatic Ketones by Capillary Electrochromatography with Polyacrylamide Monolithic Stationary Phase

作　　者：王东芝[1] 陈彦[1] 魏新华 盛筱[1] 赵先恩[3] 索有瑞[3] 尤进茂[1] 

机构地区：[1]曲阜师范大学化学科学学院,273165 [2]曲阜市第一高级中学,山东省曲阜市273100 [3]中国科学院西北高原生物研究所,青海省西宁市810001

出　　处：《曲阜师范大学学报（自然科学版）》2007年第2期84-88,共5页Journal of Qufu Normal University(Natural Science)

基　　金：国家自然科学基金资助项目(20075016)

摘　　要：在N-甲基甲酰胺与三(羟甲基)胺基甲烷(Tris)组成的混合体系中合成了聚丙烯酰胺类毛细管电色谱柱,以实验室自行合成的N,N-二乙基丙烯酰胺取代传统的丙烯酰胺,增强了固定相的疏水性,使分离更彻底.实验考察了电场强度、乙腈浓度、pH值等参数对电渗流的影响,同时对电渗流的重现性及柱寿命进行了评价,实现了7种芳香酮类(含单酮及1,2-二酮)化合物的快速基线分离.In this paper, the preparation of porous polyacrylamide-based monolithic columns via vinyl polymerization using N, N-diethylacrylamide, methylenebisacrylamide, vinylsulfonic acid and lauryl acrylate as monomers in mixtures of aqueous buffer and N-methylformamide has been developed. The effect of electric field strength, acetonitrile content, buffer concentration and buffer pH value on electroosmotic flow （EOF） and separation has been investigated. At the same time, the evaluation of EOF reproducibility and the useful life of column is elucidated. Under the optimum experimental conditions, a good baseline resolution for 7 aromatic ketones including single ketone and 1,2-diketone is obtained within 13 min.

关 键 词：毛细管电色谱 聚丙烯酰胺(N N-二乙基丙烯酰胺) 整体柱 芳香酮 

分 类 号：O657.8[理学—分析化学]