豚鼠脊髓腹角神经元线状溶酶体酶细胞化学研究及其电子探针X射线能谱分析  

CYTOCHEMICAL STUDY ON NEMATOLYSOSOME IN NEURON OF VENTRAL HORN OF THE GUINEA PIG SPINAL CORD AND ITS ELECTRON PROBE X RAY ENERGY SPECTRUM ANALYSIS

作　　者：李莉[1] 石玉秀[1] 孙桂媛[1] 

机构地区：[1]中国医科大学组织学胚胎学教研室

出　　处：《解剖学报》1996年第4期400-403,T012,13,共5页Acta Anatomica Sinica

基　　金：国学自然科学基金

摘　　要：为探讨豚鼠脊髓腹角神经元是否存在线状溶酶体及其酶细胞化学活性分布特点，用偏磷酸酶（Ｍｅｔａｐｈｏｓｐｈａｔａｓｅ，ＭＰａｓｅ）和酸性磷酸酶（Ａｃｉｄｐｈｏｓｐｈａｔａｓｅ，ＡＣＰａｓｅ）电镜细胞化学方法和电子探针Ｘ射线能谱分析技术，证实豚鼠脊髓腹角神经元存在线状溶酶体（Ｎｅｍａｔｏｌｙｓｏｓｏｍｅ，ＮＬＹ），同时于原位测定ＮＬＹ内的铅含量以反映酶活性强弱。ＭＰａｓｅ和ＡＣＰａｓｅ反应产物分布于圆形溶酶体和ＮＬＹ，同时在高尔基复合体的部分扁囊也有酶活性，表明该酶是在高尔基复合体上加工后输送至溶酶体。在神经元胞体、突起及突触前成分中均有呈ＭＰａｓｅ阳性和ＡＣＰａｓｅ阳性的ＮＬＹ的分布，并和线粒体紧密相贴，提示酶是由线粒体提供能量的ＮＬＹ从胞体输送到神经终末，可能参与神经递质的降解及神经元代谢物质的处理。电子探针Ｘ射线能谱分析测定结果，ＭＰａｓｅ活性强于ＡＣＰａｓｅ活性。y means of cytochemistry and electronic probe X ray energy spectrum analysis, the existence of nematolysosome (NLY) with ACPase avtivity and MPase (metaphosphatase) activity in the soma, process and presynaptic element of neuron in ventral horn of the guinea pig spinal cord was certificated. The ACPase positive product and MPase positive product were observed to distribute in spherical lysosome, NLY and parts of Golgi saccules, suggesting the two enzymes were processed in Golgi saccules before being transported to lysosomes. NLYs attached to mitochondria closely were found to distribute in the soma, process and presynaptic element, indicating that the enzymes were transported from the soma to nerve endings by NLY depending on mitochondria supplying energy for movement. Our findings implied that NLY might be related to the decomposition of neurotransmitters and the disposition of metabolites in neuron. The results of X ray energy spectrum analysis indicated that the activity of MPase was higher than that of ACPase, this result completely complied with the observation that the number of MPase positive NLY was more than that of ACPase positive NLY. Our results showed that, as a lysosomal marker enzyme, MPase was more sensitive than ACPase in the neuron of ventral horn of the guinea pig spinal cord.

关 键 词：线状溶酶体 脊髓腹角 神经元 酶细胞化学 

分 类 号：R322.81[医药卫生—人体解剖和组织胚胎学] R329.26[医药卫生—基础医学]