机械牵张对大鼠心室肌连接蛋白43及神经钙粘附素表达的影响  

Effect of mechanical stretch on the mRNA expression of Connexin 43 and N-cadherine in rat ventricular myocytes

作　　者：闵敏[1] 程龙献[1] 廖玉华[1] 

机构地区：[1]华中科技大学同济医学院附属协和医院心内科,武汉430030

出　　处：《广东医学院学报》2007年第1期4-6,共3页Journal of Guangdong Medical College

基　　金：国家自然科学基金(No30270559)

摘　　要：目的通过观察机械牵张后大鼠左心室肌连接蛋白43(Cx43)及神经钙粘附素(N-cadherine)的改变来研究机械牵张对大鼠心室肌Cx43及N-cadherine表达的影响。方法采用膨胀球囊持续牵张Langendorff方法灌流的离体大鼠左心室心脏模型,从左心室游离壁心肌提取总核糖核酸(RNA),用逆转录-聚合酶链氏反应(RT-PCR)方法检测心室肌Cx43及N-cadherine的信使RNA(mRNA)。结果经过50 min持续牵张的大鼠左心室游离壁心肌Cx43 mRNA水平(2.105±0.480)及N-cadherine mRNA水平(1.650±0.233)较对照组(0.905±0.132,0.891±0.082)明显增高(P<0.01)。结论机械牵张使心肌细胞间连接相关蛋白表达增加,从而提高电流在相邻心肌细胞间的传导速度,可能导致心律失常的形成。Objective To observe the effect of mechanical stretch on expression of genes Connexin 43 and N-cadherine in rat ventricular myocytes. Methods The isolated rat hearts were perfused with Langendorff technique where the balloon in the left ventricle was inflated to statically stretch the left ventricular myocardium. Total RNA was extracted from each free wall of left ventricles and the rnRNA expression of Connexin 43 and N-cadherlne were analyzed with semiquantitative RT-PCR. Results The rnRNA levels of Cx43 and N-cadherine were markedly increased in the static stretch groups （2. 105 ＋ 0. 480 for Cx43, 1. 650 ＋ 0. 233 for N-cadherlne, respectively） compared with the controls （0. 905 ± 0. 132 for Cx43, 0. 891±0. 082 for N-cadherlne, respectively） （P 〈 0.01）at 50 minute point after the static stretch was started. Conclusion Mechanical stretch might increase the expression of Connexin 43 and N-cadherine genes whose proteins form electrical andmechanical junctions that enhance the propagation velocity of electronic current among myocytes, which may explain to some degree the cause of arrhythmogenesis in myocardium.

关 键 词：牵张 心律失常 大鼠左心室肌 连接蛋白43 神经钙粘附素 

分 类 号：R341[医药卫生—基础医学]