高效抗逆转录病毒治疗对AIDS患者MCP-1和MSP水平的影响  

Impact of highly active antiretroviral therapy on plasma MCP-1 and MSP in AIDS patients

作　　者：姚航平[1] 靳昌忠[1] 张福杰[2] 冯磊[3] 魏红山[4] 吴灵娇[1] 郜桂菊[4] Armin Bader Norbert Brockmeyer 吴南屏[1] 

机构地区：[1]浙江大学医学院附属第一医院传染病研究所 [2]中国疾病预防控制中心性病艾滋病预防控制中心,北京100050 [3]浙江大学医学院基础医学系,浙江杭州310058 [4]北京地坛医院传染科,北京100011 [5]德国鲁尔大学约瑟夫艾滋病研究中心

出　　处：《浙江大学学报（医学版）》2007年第2期174-178,共5页Journal of Zhejiang University(Medical Sciences)

基　　金：国家"十五"攻关项目;项目编号:2004BA719A10

摘　　要：目的:研究高效抗逆转录病毒治疗(HAART)对AIDS患者血清中MCP-1、MSP水平的影响。方法:采用ELISA法检测40例经HAART3个月的国内AIDS患者及84例经HAART3至6年的德国患者血清MCP-1、MSP的水平,荧光定量PCR测定HIV-RNA滴度,流式细胞术检测CD4+T细胞计数。结果:HIV-1感染者血清MCP-1的水平显著高于正常人群(P<0.01),MSP则显著低于正常人群(P<0.01);经短期HAART(3月)后,MCP-1水平下降(P<0.01),MSP无明显改变;长期HAART(3至6年)后MCP-1水平显著升高(P<0.01),而MSP显著下降(P<0.01),MCP-1和MSP水平的变化呈负相关(r=-0.99,P=0.029)。结论:MCP-1、MSP与AIDS病程进展有密切关系,HAART对AIDS患者MCP-1、MSP水平有显著的改变,进而改善患者病程进展。Objective： To study the effect of highly active antiretroviral therapy （HAART） on plasma levels of MSP and MCP-1 in AIDS patients. Methods： Forty Chinese AIDS patients were treated with HAART for 3 months and 84 German AIDS patients with HAART for 3 to 6 years.The pre-treatment and post-treatment plasma levels of MSP and MCP-1 were measured by enzyme-linked immunosorbent assay （ELISA）,and their correlations with CD4＋ cell counts and viral loads were analyzed. Results： The mean levels of MCP-1 were significantly higher and MSP were significantly lower in HIV-infected patients compared with the HIV-negative controls （P〈 0. 01）. After HAART for three months,there were no significant changes in the levels of these cytokines. But after long-term HAART （for 3 to 6 y）,the level of MCP-1 was increased and that of MSP decreased significantly （P〈0. 01）. There was a negative correlation between MSP and MCP-1 levels,and the same for MSP level and CD4＋cell counts; while there was a positive correlation between MCP-1 levels and CD4＋ cell counts. Conclusion： The changed plasma levels of MSP and MCP-1 are associated with HIV-1 infection and HAART may reverse the levels of these two cytokines.

关 键 词：获得性免疫缺陷综合征/药物疗法 抗逆转录病毒治疗 高效/方法 人类免疫缺陷病毒 巨噬细胞刺激蛋白 巨噬细胞趋化蛋白 

分 类 号：R512.91[医药卫生—内科学]