中国阿昌族5个X-STR位点多态性研究  

Polymorphism of five X-STR loci in Chinese Achang ethnic group

作　　者：顾珊智[1] 仲靖芳[1] 沐志强 李生斌[1] 

机构地区：[1]西安交通大学教育部环境与疾病相关基因重点实验室,陕西西安710061 [2]西安市公安局碑林分局,陕西西安710000

出　　处：《浙江大学学报（医学版）》2007年第2期185-190,共6页Journal of Zhejiang University(Medical Sciences)

基　　金：国家自然科学基金资助项目(39970401)

摘　　要：目的:调查X染色体上5个STR位点(DXS7130、DXS7132、DXS7133、DXS6804和DXS8378)在中国阿昌族群体的遗传多态性及其法医学方面的应用价值。方法:用PCR和变性聚丙烯酰胺凝胶电泳结合银染显带技术,检测100名阿昌族无关个体上述5个位点的等位基因频率及基因型分布,计算法医学常用参数,并对女性进行Hardy-Weinberg平衡检验。结果:5个位点在中国阿昌族群体中均具有遗传多态性,女性的基因型频率分布经χ2检验均符合Hardy-Weinberg平衡定律。结论:研究结果丰富了中华民族基因数据库,可以应用于法医学个体识别和亲权鉴定及群体遗传学研究。Objective： To investigate the genetic polymorphism and applied value in forensic medicine of five short tandem repeats （STRs） loci （DXS7130,DXS7132,DXS7133, DXS6804 and DXS8378） on X-chromosome in Achang ethnic group. Methods： Allele frequency and genotype distribution of five X-STR loci among 100 unrelated individuals from Achang ethnic group were analyzed by PCR following polyacrylamide gel electrophoresis and silver staining. Hardy -Weinberg equilibrium of females was tested and forensic parameters were calculated. Results： All the five STR loci were polymorphic in Achang ethnic group. Chi-square test indicated that genotype distribution of females was in accordance with Hardy-Weinberg equilibrium. Conclusion： These results enrich Chinese genetic database and can be applied to individual identification ,paternity testing and population genetics.

关 键 词：X染色体 串联重复序列 遗传学 群体 遗传多态性 中国阿昌族 

分 类 号：Q987[生物学—遗传学]