高效液相色谱法测定垂盆草药材中小麦黄素苷的含量  

RP-HPLC Determination of Tricin-7-O-D-glucoside in Herba Sedi

作　　者：张思巨[1] 王跃生[1] 刘丽[1] 魏太明[2] 闫玉凝[2] 

机构地区：[1]中国中医科学院中药研究所,北京100700 [2]北京中医药大学药学院,北京100102

出　　处：《中国实验方剂学杂志》2007年第4期4-5,共2页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家重点科技项目改革计划(99-929-01-034)

摘　　要：目的:建立反相高效液相色谱法测定垂盆草药材中小麦黄素苷的含量。方法:采用YMC-J’Sphere ODS-H80(4μm,150 mm×4.6 mm)等色谱柱;以异丙醇-水-冰醋酸(15∶8∶2)为流动相;检测波长350 nm。结果:小麦黄素苷在0.099-0.99μg范围内峰面积与进样量呈良好的线性关系,相关系数r=0.999 8;平均回收率为97.04%。结论:该法简便可行,结果准确可靠,适用于药材质量控制的定量方法。Objective： To establish a RP-HPLC method for determination of tricin-7-O-β-D-glucoside in Herba sedi .Methods：YMC-J＇ Sphere ODS-H80（150 mm × 4.6 mm,4 μm） and YMC-pack ODS-A colunm（250 mm × 4.6mm, 5 μm） were adopted. The mobile phase consisted of （CH3）2CHOH-H2O-HAC（15：8：2）, the detection wavelength was 350 nm. Results： tricin-7-O-β-D-glucoside and other components in herba sedi can be separated, the calibration curve was linear in the range of 0.099 - 0.99 μg with correlation coefficient 0.999 9. The average recovery was 97.04 % with RSD 0.81% （ n = 5）. Conclusion. The method is simple, rapid, reliable. It can be used as quantitative determination for quality control of Herba sedi.

关 键 词：垂盆草 小麦黄素苷 高效液相色谱法 

分 类 号：R284.1[医药卫生—中药学]