中药复方“别敏”治疗呼吸道变应性疾病作用机制的实验研究  

Experimental Study of the Chinese Herbal Formulation Biminne in Treating Airway Allergic Disease

作　　者：张素琴[1] 张新民[1] 胡国让[2] 刘闰红[1] 陈伟华[1] 

机构地区：[1]复旦大学附属华山医院中西医结合研究所,上海200040 [2]澳大利亚悉尼大学Concord医院免疫与变态反应科

出　　处：《中国实验方剂学杂志》2007年第4期37-40,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：上海市科委攻关项目(02DZ19128)

摘　　要：目的:探讨中药复方“别敏”治疗呼吸道变应性疾病的作用机制。方法:将豚鼠分为5组:正常组,模型组,“别敏”高、中、低剂量组。于实验第1天用10%卵蛋白腹腔注射致敏豚鼠,于第15天用5%卵蛋白雾化吸入诱发哮喘发作,观察各组豚鼠引喘潜伏期,外周血、支气管肺泡灌洗液(BALF)中嗜酸性粒细胞和IL-5水平变化,脾淋巴细胞增殖反应及血清皮质酮水平变化。结果:模型组豚鼠外周血和BALF中嗜酸性粒细胞、血清IL-5以及血清皮质酮水平明显高于正常组(P<0.05)。中药复方“别敏”能够明显延长致敏豚鼠引喘潜伏期(P<0.05),抑制外周血及BALF中嗜酸性粒细胞水平升高(P<0.05),抑制脾淋巴细胞的增殖反应特别是高剂量组(P<0.05),降低血清IL-5水平尤其是高、中剂量组(P<0.05)。结论:中药复方“别敏”能有效防治呼吸道变应性疾病,其机制可能通过调节淋巴细胞功能,降低IL-5水平,降低气道和外周血嗜酸性粒细胞的数量而起作用。Objective：To investigate the mechanism of the Chinese herbal formulation Biminne in treating airway allergic disease. Methods： We used guinea pig model of allergic disease to study the effects of the Chinese herbal formulation Biminne on the airway allergic disease. The guinea pigs were divided to 5 groups：normal group, model group, high-dose group, middle-dose group, low-dose group. On day 1 the guinea pigs were sensitized by injecting intraperitoneally with 10% ovalbumin（OVA）, and on day 15 the sensitized guinea pigs were subjected to 5% OVA inhalation challenge. Then we observed the index such as the eosinophil and IL-5 levels in blood and bronchial airway lavaging fluid（BALF）, corticosteroid level in blood and the spleen cell proliferation responsiveness. Results： Compared with normal group, the eosinophil number in blood and BALF, and IL-5 level in blood increased significantly in model group （ P 〈 0.05）, But Biminne could significantly prolong the time when the asthma attack happened, attenuated the increase in the eosinophil and IL-5 levels, especially the hige-dose and middle-dose groups（ P 〈 0.05）, and also suppressed the proliferation of spleen cells, especially the hige-dose group （ P 〈 0.05 ） .Conclusion： The results suggest that the Chinese herbal formulation Biminne has some antiasthmatic effect, partly mediated by attenuating eosinophil and IL-5 levels and suppressing the spleen cell proliferation in OVA-sensitived guinea pigs. Thus the Chinese herbal formulation Bimnne may be useful in preventing and treating allergic disease.

关 键 词：变态反应 呼吸道 嗜酸性粒细胞 白介素-5 别敏 

分 类 号：R285.5[医药卫生—中药学]