断肢大鼠血浆及血管壁中内皮素变化的意义  

Changes of endothelin-1 concentration of blood and local vascular wall in amputated rats

作　　者：谭文秀[1] 金惠铭[1] 顾玉东[2] 

机构地区：[1]上海医科大学病理生理教研室,200032 [2]上海医科大学华山医院手外科

出　　处：《中华手外科杂志》1996年第A00期38-41,共4页Chinese Journal of Hand Surgery

基　　金：本课题为美国中华医学基金及中国自然科学基金资助项目

摘　　要：目的:研究断肢后血管痉挛与内皮素-1(endothelin ET)的关系,和ET在创伤性血管痉挛发生发展中的作用。方法:在158只断肢SD大鼠中用激光多普勒微循环血流仪测定局部血流量,放射免疫分析法测定血液及局部血管壁ET的含量。结果:(1)局部血流量逐渐下降,以断肢后2小时降到最低,以后又逐渐升高。(2)颈总动脉中ET的含量与断肢前比,无明显变化(P＞0.05)。(3)局部股动脉血管壁ET含量较断肢前逐渐升高,并于断肢后2小时达到峰值,此时与断肢前比,两者差异有显著性意义(P＜0.01),以后又逐渐下降。(4)断肢后2小时,近断端股动脉血管壁ET含量较远断端显著增高,两者差异有显著性意义(P＜0.05)。结论:ET是一个强烈的血管痉挛剂,它在断肢后局部血管痉挛的发生发展中起着重要作用。<Abatract>Objective: To study the changes of local hemodynamics and the importance of endothelin-1 (ET-I) during amputation. Methods: To use Laser Doppler Flowmeter for detecting the local blood perfu-sion, radioimmunoassay for determining ET-I concentration of blood and local vascular wall in 158 SD rats. Results: (1) The local blood perfusion after amputation decreased, reaching its lowest value at 2h postamputation, and subsequently increased slowly. (2)ET-I concentration in plasma of common carotid artery blood after amputation was comparable to that before amputation (P>0.05). (3)ET-1 concentration of femoral artery wall after amputation increased, reaching peak value at 2h postamputation, having significantly difference in comparison with that before amputation (P<0.01), and then decreased slowly. (4) ET-1 concentration of vascular wall of proximal part of femoral artery near amputation was higher than that of distal part at 2h postamputation (P<0.01). Conclusions: The time-course change and distribution of ET-1 were elucidated. ET-1 is important in pathogenesis of local vascular spasm.

关 键 词：内皮素 血管痉挛 断肢 大鼠 

分 类 号：R658[医药卫生—外科学]