萝卜花药愈伤组织诱导与分化影响因素的研究  

Study on Factors Affecting the Induction and Differentiation of Anther Callus in Radish

作　　者：朱献文[1] 龚义勤[2] 柳李旺[1] 黄丹琼[1] 赵艳玲[1] 汪隆植[2] 

机构地区：[1]南京农业大学作物遗传与种质创新国家重点实验室,中国江苏南京210095 [2]南京农业大学园艺学院,中国江苏南京210095

出　　处：《生命科学研究》2007年第1期90-94,共5页Life Science Research

基　　金：国家自然科学基金资助项目(30571193);江苏省自然科学基金资助项目(创新人才BK2004418);江苏省高技术研究计划资助项目(BG2005314)

摘　　要：以5个萝卜品系为材料探讨了不同培养方式、激素与基因型对花药愈伤组织诱导的影响.结果表明固液双层培养较固体与液体培养效果好,0.2mg/L2,4-D与4.0mg/LKT+0.2mg/LNAA对花药愈伤诱导率较高,4.0mg/LKT+0.2mg/LNAA的激素组合愈伤组织生长快,NAA为2.0mg/L时根分化明显,各种激素处理均未见芽的分化.5个品系中NAU-Yb-03与NAU-Dy-13-03对激素反应较为敏感;NAU-Dq-03在所有的处理中均未见愈伤组织的形成.Five advanced lines were employed in researching the effect of different culture methods, hormones and genotypes on the induction of anther calli in radish. The results showed that liquid/solid bi-layer culture medium was better than liquid medium and solid medium, and when the concentration of 2, 4-D was 0. 2 mg/L or the concentration of KT and NAA was 4.0 mg/L and 0. 2 mg/L, respectively, the higher ratio of anther calli induction was obtained. The calli grew fast on the medium supplemented with 4.0 mg/L KT and 0. 2 mg/L NAA. The roots obviously differentiated from it when NAA was 2.0 mg/L, but shoots were not obtained in all treatments. Of the five lines, ＂NAU-Yb-03＂ and ＂NAU-Dy-13-03＂ were more sensitive to hormones than the others, and anther calli could not been induced from all treatments in ＂NAU-Dq-03＂.

关 键 词：萝卜 花药 愈伤 培养方式 激素 基因型 

分 类 号：S631.1[农业科学—蔬菜学]