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出 处:《中华中医药学刊》2007年第4期812-813,共2页Chinese Archives of Traditional Chinese Medicine
摘 要:目的:选择提取三白草中总黄酮的最佳方法。方法:采用紫外分光光度外法测定总黄酮含量,并且利用正交实验法对影响三白草提取效果的因素进行了研究。结果:总黄酮最佳提取工艺为以药材粉碎过20目筛后,加20倍量70%乙醇回流4h提取2次,第1次加12倍量乙醇,提取2h,第2次加8倍量乙醇,提取2h。结论:该方法可用于三白草中总黄酮的提取。Objective: To optimize extracting parameters of total flavonoids from Saururus chinensis (Lour) Bail Methods: The total favoniods was detenmined by UV spectrophotometry, and use the orthogonal design as guided by the conent of flavonoids in Saururus chinensis(Lour) Bail present in the extract, Results: The best condition of extraction was decided : reflux extraction with the 20 - fold 70% alcohol for 2 times, 2 hours for each time, 12 - fold alcohd for the first time,and 8- fold alcohol for the second time. Conclusion. This method can be used for the extraction of the total flavonoid in Saururus chinensis(Lout) Bail,
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